Abstract

Nerium odorum is an imperative species with worldwide therapeutic and commercial uses. N. odorum has been considered as a potentially important plant for industrial and pharmacological applications. Callus induction potential of N. odorum was assessed from leaf explants cultured on Murashige and Skoog’s medium using different plant hormonal treatments. A range of different concentrations of 6-benzylaminopurine, 1-naphthaleneacetic acid, 2,4-dichlorophenoxyacetic acid (2,4-D), indole-3-acetic acid (IAA), and kinetin (0.5–5.0 mg/L) have been incorporated in the culture medium to investigate the biomass, polyphenols production and oxidizing and antibacterial activity of the callus. The results showed highest callus induction at 2.0 mg/L in growth hormone combination of IAA and 2,4-D. The total phenolic content was 92.14 mg GA/g dry weight (DW). In addition, the 2,2-diphenyl-1- picrylhydrazyl activity and 2,2’-azinobis-(3-ethylbenzthiazoline-6-sulfonic acid) activities were 46.7 µg/mL DW and 19.9 µg/mLDW, respectively. Interestingly, the explants produced hard calli using combination of IAA and 2,4- D presented a higher phenolic content and antioxidant capacity when compared to individual growth hormones of either IAA or 2,4-D. Altogether, these results demonstrate the extraordinary effect of different growth hormones on polyphenols production, antioxidant, and antibacterial activities in N. odorum.

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