Effect of Low Frequency Electromagnetic Fields (LF-EMFs) on c-myc Oncogene Expression Level in Peripheral Blood Mononuclear Cells (PBMCs)
Journal Title: Biotechnology Journal International - Year 2014, Vol 4, Issue 12
Abstract
Low frequency electromagnetic fields (LF-EMFs) which can be generated from homes and workplaces appliances can lead to alteration in oncogenes causing cancer diseases such as leukemia, nervous system tumors, lymphoma and breast cancer. To investigate the effect of LF-EMFs on c-myc oncogene expression level, primary cell culture of human peripheral blood mononuclear cells (PBMCs) were exposed to AC (50 Hz) electromagnetic flux density: 0.37mT, 0.82mT, 1.22mT, 1.68mT, 2.1mT, 2.47mT, 2.85mT, 3.33mT, 3.72mT, 3.92mT, 4.32mT and 4.67mT using exposure unit. After four days of exposure, when initial changes in cell viability, morphology and count between exposed and unexposed cells were noted, total RNA was extracted to evaluate the expression level of c-myc oncogene by quantitative real time PCR (qRT-PCR). The results showed that c-myc oncogene expression level began to increase gradually from value 1.69% at 0.82mT reaching to the maximum expression level value 4.65% at 4.67mT. This result refers to an increasing in LF-EMFs exposure offset by an increasing in c-myc oncogene expression level, affecting different cellular functions. This study indicates that LF-EMF is environmental pollution effects on the expression level of oncogenes which increase the risk of human cancer diseases.
Authors and Affiliations
Salah Abdel-Rahman, Yehia Mustafa, Sanaa Riad, Maha El-Demellawy, Sarah El-Messeiry
Keywords
Related Articles
- EP ID EP237780
- DOI 10.9734/BBJ/2014/14106
- Views 120
- Downloads 0
Haploid Plant Regeneration from Unpollinated Ovules of Cucumis melo L. Var. Conomon cv. Mudicode
Major objective of the present work was to induce haploid plants in Cucumis melo var. conomon cv. Mudicode (Common name: Kani vellari). Ovules of Kani vellari plants were collected one day before anthesis and were cultur...
Detection and Analysis of the Random Mutagenesis Site(s) of Xylanase Gene from Mutants of Bacillus subtilis subsp. spizizenii ATCC 6633
Aims: A total of five mutant strains of Bacillus subtilis subsp. spizizenii ATCC 6633 designated as the MXB 1, MXB 2, MXB 3, MXB 4 and MXB 5 were developed using random mutagenesis of ethyl methane sulfonate (EMS) and ac...
In vitro and In vivo Effects of the Leaf Extracts of Cassia tora and Cassia sophera in Reducing the Cytotoxicity and Angiogenesis
Aim: Consumption of C. tora and C. sophera of family caesalpinaceae as leafy vegetables by local folklore has initiated this study to investigate the cytotoxic effect and antiangiogenic effect of their leaf extracts on h...
Effect of BAP and IBA on in vitro Regeneration of Local Banana Variety of Sabri
The experiment was carried out to study the effect of benzylaminopurine (BAP) (1.0, 2.0, 3.0, 4.0 and 5.0 mg/L) and indole-3-butyric acid (IBA) (0.5, 1.0, 1.5, 2.0 and 2.5 mg/L) for in vitro regeneration of sabri variety...
Properties of Aspergillus flavus Cellulase Produced from Solid State Fermentation of Brewers’ Spent Grain (BSG) as Substrate
The production of cellulase from Aspergillus flavus isolated from Brewers’ Spent Grain (BSG) undergoing bio-deterioration was carried out using solid state fermentation method. Out of a total of four fungal isolates with...