Effect of methyl jasmonate and salicylic acid on noradrenalin accumulation in hairy roots of Portulaca oleracea L
Journal Title: International Research Journal of Applied and Basic Sciences - Year 2012, Vol 3, Issue 1
Abstract
Portulaca oleracea L. is a medicinal plant find in Europe and Asia. This plant contains valuable secondary metabolite such as noradrenalin. Naturally the wild plant produce small amount of this secondary metabolites so induction of hairy roots can be useful to increase its production. In our research Agrobacterium rhizogenes strain AR15834 was used to induce hairy roots in the two-week leaf explants of P. oleracea. PCR analysis with specific primers rolB gene was performed to confirm the transgenic hairy roots production. PCR analysis results showed diagnostic bands with size 780 bp related to specific reproduction of rolB gene, so the transgenic hairy roots was confirmed. The effect of two chemical elicitors, salicylic acid and methyl jasmonate, on the production of noradrenalin in P. oleracea hairy roots was examined. Methyl jasmonate was found to increase the production of noradrenalin. The optimal methyl jasmonate dose was between 100um and 200um for hairy roots harvested 48h after elicitation. After 48h of induction with 200um methyl jasmonate, an eightfold increase in the level of noradrenalin was observed in compared with control cultures.
Authors and Affiliations
Kiana Pirian| M.Sc student, Department of Biotechnology, Faculty of Agriculture, Bu-Ali sina University Hamedan, Iran., Khosro Piri*| Associate Professor, Department of Biotechnology, Faculty of Agriculture, Bu-Ali sina University Hamedan, Iran.khpiri@gmail.com
Keywords
Related Articles
- EP ID EP4899
- DOI -
- Views 540
- Downloads 32
The Effective Motivations OnEarning Management
Users of financial statements and other accounting reports, Potential investors, especially if associated with the procedure and how to manipulate profits by managers earn more, achieve more informed economic decisions....
Study effects of leadership styles on creativity behavior of stuffs (Case study: welfare organization of Abhar city)
This research aims to investigate effects of leadership styles on creativity of stuffs. Based on goal this study is applied, based on subject features is descriptive, and based on methodology is survey. Statistical socie...
Natural convective boundary-layer flow of a nanofluid past a vertical plate with heat flux condition
The paper analytically investigated the double-diffusive natural convective boundarylayer flow of a nanofluid past a vertical plate with heat flux condition. The diffusive model used for nanofluid included the effects of...
The relationship between knowledge management and entrepreneurship
In modern and current information-oriented world, knowledge management plays main function and important role in organizations and society, because employing this concept leads to make capability in organizations till mo...
A Survey on 24 Hours Short Term Rainfall, Bandar-e Anzali
Short term rainfalls are a form of liquid precipitation that rain suddenly, and interrupt quickly too. Awareness about rainfall occurrence and its harmful impacts, significantly, will help performing developmental projec...