Effect of Omega-3 Fatty Acids on Erythrocyte Membrane in Diabetic Rats
Journal Title: Macedonian Journal of Medical Sciences (MJMS) - Year 2011, Vol 4, Issue 3
Abstract
Background: Diabetes mellitus is a metabolic disease characterized by chronic hyperglycemia resulting from defects in insulin secretion, almost always with a major contribution from insulin resistance which may be affected by cell membrane fatty acids and phospholipids fractions. Aim: To evaluate the effects of omega-3 fatty acids on erythrocyte membrane and also in decreasing oxidative stress in diabetic rats. Materials and Methods: Sixty healthy male albino rats weighting 180-200 g divided into five groups included: control, Omega-3, diabetic, prophylactic and treated groups. Fasting blood samples were collected from all groups for determination of fasting blood sugar, plasma insulin, erythrocyte membrane lipid profile, phospholipids fractions by HPLC, plasma and erythrocyte membrane lipid peroxidation product (malondialdehyde) and reduced glutathione. Results: Fasting blood sugar and plasma insulin sensitivity were improved by Omega-3 administration, also reduced glutathione improved by Omega-3 in both erythrocyte membrane and plasma, while, malondialdehyde decreased indicated the decrease of oxidative stress by Omega-3 administration. Conclusion: Omega-3 supplementation increased insulin sensitivity by improving cell membrane contents especially phospholipids fractions.
Authors and Affiliations
Jihan Hussein| Medical Biochemistry Department, National Research Centre, Doki, Giza, 12311, Egypt, Ehab Mostafa| Biochemistry Department ,Faculty of Science, Tanta University, Tanta, Egypt, Maha El-Waseef| Medical Biochemistry Department, National Research Centre, Doki, Giza, 12311, Egypt, Zakarya El-Khayat| Medical Biochemistry Department, National Research Centre, Doki, Giza, 12311, Egypt, Ehsan Badawy| Medical Biochemistry Department, National Research Centre, Doki, Giza, 12311, Egypt, Dalia Medhat| Medical Biochemistry Department, National Research Centre, Doki, Giza, 12311, Egypt
Keywords
Related Articles
- EP ID EP8810
- DOI 10.3889/MJMS.1857-5773.2011.0180
- Views 358
- Downloads 16
Analysis of Macedonian Medical Scientific Papers in the Scopus Database
Aim: The aim of this study was to analyze medical scientific papers in the Republic of Macedonia indexed in the Scopus database. Material and Methods: A search of the Scopus database was performed on February 23, 2013...
Association between Anthropometric Parameters and CardioMetabolic Disease Risk Factors Among Obese Children and Adolescents
Background: Childhood obesity increases risk for developing cardio-metabolic diseases. Objectives: to investigate the relation of some anthropometric parameters with cardio-metabolic disease risk factors in obese child...
Presence of Circulating Lactate DehydrogenaseImmunoglobulin G (LDH-IgG) Complex in the Sera of PostBurn Patient
Lactate dehydrogenase (LDH) is an enzyme that belongs to the class oxidoreductase. It actively participates in the inter-conversion of lactate to pyruvate during anaerobic glycolysis in cell cytoplasm. Available litera...
Macedonian Medical Master of Science (MSc) Theses Defended in 2007
A Master of Science (MSc) (Latin: Magister Scientiæ; abbreviated MSc, M.Sc., M.S. or S.M.) is a postgraduate academic master’s degree awarded by universities in a large number of countries. The degree is typically s...
Bacteriology of Wound - Clinical Utility of Gram Stain Microscopy and the Correlation with Culture
Aim: To determine the most common bacteria isolated from wound specimens and to compare those culture results to Gram stain slides. Material and methods: A total of 1970 specimens from 1788 patients, treated in the Uni...