Effect of sodium chloride on the ascorbate content in Arabidopsis thaliana
Journal Title: Naukovyi visnyk Chernivetskoho universytetu. Biolohiia (Biolohichni systemy) - Year 2017, Vol 9, Issue 2
Abstract
Soil salinization is one of the main factors limiting the productivity of crop production. The increase of salt (primarily sodium chloride) content in the plants causes saline stress, one of the manifestations of which is the elevated formation of reactive oxygen species (ROS) in chloroplasts and peroxisomes. This is due to the closure of stomata, the decreased CO2 availability and the inhibition of Calvin cycle reactions, which in turn leads to over-reduction of the electron transport chain components in the chloroplasts and activation of photorespiration. ROS oxidize the molecules of proteins, lipids, nucleic acids, disrupt the permeability of the plasma membranes and the redox homeostasis of the cell. Low molecular weight antioxidants, in particular ascorbate (vitamin C), play a key role in preventing oxidative damage. Ascorbate protects plants from oxidative stress elicited by drought, ozone, intensive lighting, etc. However, the role of ascorbate in the early stage of the plant cell response to saline stress is still not clear enough. Therefore, in this paper, we study the changes in the content of the reduced (Asc) and oxidized (DHA) forms of vitamin C in the model plant Arabidopsis thaliana in response to stress caused by the rapid flow of sodium chloride into the leaf tissues.In this study, we used 4.5-5 week-old A. thaliana plants of the Columbia 0 ecotype. The plants were grown at a constant temperature of +20°С and an illumination of 2000 Lux under a 16-hour photoperiod. For the stress treatment, the aboveground part of the plant was separated from the root system and the cutting place immersed in liquid Murashige and Skoog medium supplemented with 50, 100 and 200 mM sodium chloride. The specimens were incubated in the light or in the dark for 4 and 8 hours. It was found that in control plants incubated in the dark for 4 and 8 hours in medium without added sodium chloride, Asc content decreased by 19% and 13%, respectively, and the DHA content increased by 61% and 48%. As a result, the total Asc + DHA pool remained the same as in intact plants, but the proportion of the oxidized form increased. The content of Asc decreased under light conditions in control samples compared to intact plants, as was the case in darkness. However, DHA content did not increase. Treatment with sodium chloride in the dark did not result in changes in Asc concentration in the leaves after 4 hours. After 8 hours Asc content decreased by 15% in the 200 mM NaCl treatment. The total pool of Asc + DHA decreased by 24%. Asc content remained unchanged after 4 hours of stress treatment in light conditions. The DHA content was lower compared to control samples. After 8 hours of treatment with 200 mM NaCl, there was a decrease in Asc content by 18% and DHA by 19%. Thus, the results obtained indicate that the transfer of Arabidopsis leaves to the nutrient medium has different effects on the content of Asc and DHA depending on the lighting conditions. Salt stress leads to the depletion of the Asc + DHA pool in the leaves of Arabidopsis. This effect is already observed at an early stage of stress, both in the dark and under illumination. The decrease of the Asc + DHA pool depends on the duration of stress and the concentration of sodium chloride used for plant treatment.
Authors and Affiliations
Інна Буздуга, Ірина Панчук
Keywords
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- EP ID EP303850
- DOI 10.31861/biosystems2017.02.176
- Views 75
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