Effect of supercritical carbon dioxide on inactivation and germination of Alicyclobacillus acidoterrestris spores
Journal Title: Żywność. Nauka. Technologia. Jakość - Year 2016, Vol 23, Issue 1
Abstract
The spores of thermophilic, acidophilic bacterium Alicyclobacillus acidoterrestris are resistant to external factors and can develop in juices during storage. They produce compounds that smell like disinfectants, i.a., guaiacol, and lead to the spoilage of juices. The increasing of the degree of spore germination during the process of food preservation can contribute to their effective inactivation. The objective of the research study was to characterise the course of the germination process and the inactivation degree of Alicyclobacillus acidoterrestris spores initiated by supercritical carbon dioxide (SCCD). The spores suspended in apple juice and in buffers of pH 4.0 and pH 7.0 were treated with SCCD at a pressure of 10-60 MPa, at temperatures of 50 ÷ 75 ºC, for 20 ÷ 40 min. After 40 min SCCD treatment at 60 MPa and at 75 ºC, the count of spore germination in apple juice was 3.9 log, of which 3.4 log were inactivated. In the pH 4.0 buffer and under the same process conditions, a lower degree of germination (3.2 log) and inactivation (2.7log) was achieved. In the pH 7.0 buffer, the germination of 2.5 log was reported and the spore inactivation of 1.1 log. A significant correlation was found between the results of germination degree estimation that were obtained using a plate method and a method of measuring the decrease in optical density. The temperature of the SCCD applied turned out to be a factor to significantly impact the degree of germination and the inactivation of A. acidoterrestris spores. Along with the decrease in pH, a slight increase in the degree of spore germination was observed and a significant increase in the inactivation of the A. acidoterrestris spores treated with SCCD; the content of nutrients in apple juice further increased the degree of germination.
Authors and Affiliations
Izabela Porębska, Barbara Sokołowska, Łucja Łaniewska-Trokenheim
Keywords
Related Articles
- EP ID EP107193
- DOI 10.15193/zntj/2016/104/110
- Views 74
- Downloads 0
Temperatura przejścia szklistego i krytyczne parametry przechowywania skrobi ziemniaczanej i jej pochodnych
Celem niniejszej pracy była ocena wpływu utleniania, acetylacji oraz podwójnej modyfikacji (acetylacji i utleniania) na temperaturę przejścia szklistego (Tg) i krytyczne parametry przechowywania skrobi ziemniaczanej. Utl...
Porównanie plonowania i cech morfologicznych roślin owsa oplewionego uprawianego w siewie czystym i mieszanym na terenie powiatu sanockiego
W badaniach podjętych na terenie trzech gmin: Sanok, Zarszyn i Besko, w powiecie sanockim, analizowano w latach 2007 i 2008 produkcyjność owsa i jego mieszanki z jęczmieniem. Ocenie poddano komponenty struktury plonu o...
Zastosowanie metody NMR do analizy wiązania wody w kleikach skrobiowych
Prozdrowotne właściwości resweratrolu
Resweratrol (3,5,4’-trihydroksy-trans-stilben) jest naturalną fitoaleksyną, syntetyzowaną m.in. w skórkach winogron w odpowiedzi na infekcje grzybicze, stres oksydacyjny oraz promieniowanie UV. W wysokim stężeniu wystę...
EFFECT OF BUCKWHEAT SEED PREPARATIONS ADDED ON QUALITY OF PÂTÉS DURING STORAGE
The subject of the research were baked pâtés, which contained a high content of fat and a 5% addition of processed buckwheat seeds (with and without husk) and buckwheat flour; the control samples contained no buckwheat...