Effect of the in vitro shoot handlings of Xanthosoma sagittifolium (L.) and Colocasia esculenta (L.) Schott during multiplication stage
Journal Title: Biotecnología Vegetal - Year 2018, Vol 18, Issue 2
Abstract
To solve the limited in vitro multiplication coefficients in taro (Xanthosoma and Colocasia) several strategies are used. This work was carried out with the aim of to determine the effect of the in vitro management of shoots of both species of taro that allows to increase the multiplication coefficient in order to develop a simple method of in vitro propagation of this crop. Four cultivars of Xanthosoma (‘Mexico 1’, ‘Mexico 8’, ‘Yellow Special’, ‘Selection INIVIT’) and the cultivar ‘Cameroon 14’ of C. esculenta were used in the multiplication phase. The treatments included variants of decapitation and cut of the shoots. After 25 days of culture, the number of shoots per explant was quantified and the fresh mass was determined. The best results were achieved with treatments 6 (Decapitated 75% of the shoot and cut in the apical bud) and 7 (Decapitated 75% of the shoot, longitudinal cut and bevel of the apical bud) in all cultivars. The multiplication coefficients in both treatments varied from 3.00 to 6.98 depending on the cultivar. It is possible to increase the multiplication coefficient using other forms of cutting of the explants different from the traditional ones used (Beheaded 50% of the shoot and bevel cut).
Authors and Affiliations
Diosdada Galvez Guerra , Sergio Juan Rodríguez Morales , Marilys Milián Jiménez , Juan Ramón Galvez Guerra , Laisyn Posada-Pérez , Rafael Gómez Kosky , Adrián Rubio Cabrera , Ania Robaina Jiménez , Dion Daniels , Maricel Bauta Toledo , Jesús García Ruíz
Keywords
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