Evaluation in a greenhouse of five short mutants of the banana hybrid 'FHIA-21' (Musa AAAB) by artificial inoculation of Mycosphaerella fijiensis
Journal Title: Biotecnología Vegetal - Year 2007, Vol 7, Issue 1
Abstract
The development of methodologies that allow to evaluate great volumes of plants and to detect in an early and fast way the searched characters facilitates greatly the works of selection in the Breeding Programs of banana and plantains. Five mutants of plantain hybrid ‘FHIA-21’ (Musa AAAB), treated with physical mutagenic agents (Gamma radiations, 60 Co source), those that had been selected previously in the field by their low height were used for the studies. ‘Grande naine’ (Musa AAA) susceptible, ‘FHIA-20’ (Musa AAAB) as resistant and ‘FHIA- 21’ without irradiation were used as controls . They were multiplied in vitro and acclimatized for (60 days). The plants were inoculated with mycelia suspensions of Mycosphaerella fijiensis at a concentration of 1x 10 5 . ml -1 by means of a brush by abaxial leaf surface of the four first open leaves. Affectation degrees caused by the pathogen were evaluated after 15, 30, 45 and 60 days plants had been inoculation. The first sympthoms appeared in the cultivar ‘Grande naine’ to the 45 days plants had been inoculated . Mutant ‘IBP 14-23’ stood out with the lowest values of affectation to the damages caused by Mycosphaerella fijiensis, when compared to the rest of mutants studied, corroborating its behavior in field.
Authors and Affiliations
Idalmis Bermúdez-Caraballoso, Michel Leiva-Mora, Yelenys Alvarado-Capó, Lourdes R. García, Novisel Veitía, Mayra Acosta-Suárez
Keywords
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