Effect of two brassinosteroid analogues on the induction and regeneration of callus from Ipomoea batatas
Journal Title: Biotecnología Vegetal - Year 2003, Vol 3, Issue 3
Abstract
With the purpose of studying, in different clones, the effect of two similar brassinoesteroids in the induction of callus and the regeneration of the same ones, tuberous roots belonging to the clones Cemsa 78-354, Inivit B 90-1, Inivit B 93-1, Yabú-8 and Jewel were gathered and they were placed in flasks with water in the laboratory to induce the sprouting of the buds. Later , explants of the limbo foliar were selected and they were culture in the Murashige and Skoog (1962) culture medium with 2, 4-D (0.1 mg.l-1) and 6-BAP (0.2 mg.l-1) with the similar brassinoesteroids MH-5 and Biobras-6 at different concentrations (0.001, 0.01, 0.05 and 0.1 mg.l-1) and the employment independent brassinoesteroids analogues to induce the formation of callus. Starting from the fourth week the callus were transferred, to facilitate the regenerants induction, to the culture medium previously mentioned to which the independent brassinoesteroids analogues MH-5 and Biobras-6 and cocktails with the regulators of the growth AIA (0.05 mg.l-1) and Kinetina (0.2 mg.l-1)were added . The same ones stayed under sun light conditions to temperatures of 25 ± 2 °C, relative humidity of 80-90% and luminous intensity of 3 000-5 000 l. After four weeks, it was evaluated the morphological behavior of the callus and the regenerants. Good results were obtained in the induction of morphogenic callus , when using the regulators of the independent growth in the culture medium and when being combined with 2, 4-D (0.1 mg.l-1) and 6-BAP (0.2 mg.l-1) to the concentration of 0.01 mg.l-1 with both brassinoesteroids analogues. The regenerantes induction was more favored with the employment of the brassinoesteroids analogues Biobras-6 combined with AIA (0.05 mg.l-1) and Kinetina (0.2 mg.l-1) than when being used independent in the culture medium.
Authors and Affiliations
Orlando S. González Paneque, Miriam Nuñez Vázquez, María M. Hernández Espinosa, Juan J. Silva Pupo, Ángel Espinosa Reyes
Keywords
Related Articles
- EP ID EP697979
- DOI -
- Views 62
- Downloads 0
Ochrobactrum anthropi, contaminants of in vitro culture of sugarcane cells and tissues
The purpose of this paper was to report, for first time, the presence of Ochrobactrum anthropi as contaminant of in vitro cell and tissue culture of sugarcane. Bacteria contaminant of in vitro plants were isolated direct...
Micropropagation of Stevia rebaudiana Bertoni plants from ex vitro explants (2019)
Stevia rebaudiana Bertoni is a species recognized worldwide for its medicinal properties. The objective of this work was to micropropagate Stevia plants from ex vitro explants. Shoots of plants of this specie growing at...
In vitro regeneration of Solanum pimpinellifolium plants L. from leaf explants
Tomato is a species of agricultural importance. Besides, it is a source of vitamins, minerals and antioxidant compounds. Therefore it is essential to obtain varieties resistant to diseases. Solanum pimpinellifolium L. ‘...
Plant regeneration through somatic embryogenesis in three ethiopian Coffea arabica Lin. hybrids
Breeders at Jimma Agricultural Research Center (JARC), Ethiopia, developed three arabica coffee hybrids: Aba Buna, Ghawe and MCH 2 that exhibited heterosis over their parents. However, their distribution to farmers had...
Effect of filtered bacterial cultures with antifungal activity in vitro in the interaction Musa spp.- Mycosphaerella fijiensis
Although certain bacterial species may be in vitro antagonistic of Mycosphaerella fijiensis, as it was demonstrated, they are discrete results that have their effect on the Musa spp.-M. fijiensis interaction. The resul...