Effects of Centella asiatica Ethanolic Extract Encapsulated in Chitosan Nanoparticles on Proliferation Activity of Skin Fibroblasts and Keratinocytes, Type I and III Collagen Synthesis and Aquaporin 3 Expression In Vitro
Journal Title: Journal of Pharmaceutical and Biomedical Sciences - Year 2016, Vol 6, Issue 5
Abstract
Background The activity of skin cell proliferation, collagen synthesis and skin hydration decrease with the process of aging; therefore, the skin looks dull, dry and sagging. Aquaporin 3 (AQP3) is a key protein that plays a major role in skin cell proliferation and skin hydration. Retinoic acid (RA) is still considered for anti-aging treatment, but it frequently shows side effects, such as skin irritation. Centella asiatica (CA) formulation in chitosan nanoparticles has a promising potential as anti-aging cosmetic. Aim The aim of this study was to evaluate the effects of CA ethanolic extract encapsulated in chitosan nanoparticles (CAEE + CNP) on skin cell proliferation, collagen synthesis and AQP3 expression in vitro, when compared to RA. Methods Microculture tetrazolium assay was conducted to analyse the proliferation of normal human dermal fibroblasts (NHDF) and normal human epidermal keratinocytes (NHEK) at 24, 48 and 72 h. Type I and III collagen synthesis was evaluated at the same time points using ELISA. Aquaporin 3 expression at 24 h was evaluated using immunocytochemistry and measured quantitatively using ImageJ software. All treatments involved several concentrations of CAEE + CNP and RA through serial dilution. Results Collagen type I and III synthesis of NHDF and NHEK was neither significantly different from untreated controls nor from RA-treated cells. Nevertheless, CAEE + CNP stimulated the proliferation of both NHDF and NHEK. Additionally, AQP3 expression in both cell types was upregulated by CAEE + CNP. Conclusion CAEE + CNP is a promising formulation for anti-aging activity by inducing skin cell proliferation and AQP3 expression. The clinical trials are still needed to evaluate skin hydration in vivo.
Authors and Affiliations
Linda Yulianti Yulianti, Kusmarinah Bramono, Etik Mardliyati, Hans-Joachim Freisleben
Keywords
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