ENCAPSULATION OF INVERTASE IN BARIUM ALGINATE BEADS
Journal Title: World Journal of Pharmaceutical Research - Year 2019, Vol 8, Issue 9
Abstract
Invertase (E.C. 3.2.1.26) was encapsulated in barium alginate beads. The gelation was carried out at 30oC by stirring (360 rpm and 540 rpm) using alginate concentrations (5 g/L and 10 g/L), barium nitrate concentrations (0.1M and 0.2M), and three pH of alginate-invertase solution (4.0, 6.7 and 8.0). Invertase encapsulation yielded 75.2% and encapsulated invertase activity yielded 0.249 U/mL. These results were obtained using beads produced with 10 g/L alginate (SG800) and 0.1M barium nitrate at pH 4.0, 30oC and 540 rpm. The kinetic constants for soluble and encapsulated invertase, calculated using Hanes-Woolf’s plot, were KM = 52.9 mM, Vmax = 0.368 U/mL, (KM)encap = 7.51 mM and (Vmax)encap = 0.269 U/mL. The beads were reused three times batchwise without significant loss of invertase activity.
Authors and Affiliations
Michele Vitolo
Keywords
Related Articles
- EP ID EP673086
- DOI -
- Views 150
- Downloads 0
IMPROVED GLUCOSE TOLERANCE WITH A POLYHERBAL FORMULATION OF COLOCASIA ESCULENTA TUBERS AND ALLIUM SATIVUM CLOVES
Background: Colocasia esculenta and Allium sativum are cultivated in Bangladesh, respectively, for consumption of tubers as vegetable and use of cloves as spice. Both plant parts have previously been reported to have ant...
AYURVEDA AND PSYCHOLOGY
The name Ayurveda was given to the ancient healing tradition of India. It is often translated as the "science of life," from ayus (life) and veda (knowledge). It is an ancient science of body, mind and spirit which has s...
BACTERIAL DISEASES: HUMANS VS MICROORGANISMS
Bacteria, one among the group of microorganisms like virus, protozoa and fungi, is universally present in nature. It is in air, water and soil, the basic elements of life closely associated with all living organisms. Pre...
DETERMINATION OF BETA- CAROTENE AND VITAMIN A [RETINOL] IN PALM OIL FROM TWO VARIETIES OF PALM FRUITS (ELAEIS GUINEESIS TENERA AND DURA)
The two varieties of palm fruits, Elaeis guineansis (Tenera) and Elaeis guineensis (Dura) obtained from Ogbeke, Agbani in Enugu state was carried out to determine the Beta-carotene and Vitamin content using a spectrophot...
PHYTOCHEMICAL SCREENING AND ANTIBACTERIAL ACTIVITY OF THE CRUDE EXTRACT AND FRACTIONS OF TAPINANTHUS GLOBIFERUS LEAVES ON THE BACTERIAL ISOLATES OF WOUND
Background: Tapinanthus globiferus (Mistle toe, Kauchi) is a medicinal herb used in the treatment of many ailments ranging from infectious to non infectious diseases. Objective: To investigate the effect of the antibacte...