ENDOPHYTES FROM THE AQUATIC PLANT NELUMBO NUCIFERA: DIVERSITY PROFILE AND ACTIVITY CHARACTERIZATION
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2016, Vol 8, Issue 1
Abstract
Objective: Endophytes represent a niche habitat for the study of novel bio-and chemo diversity. Nelumbo nucifera is an aquatic plant that has not been characterized for endophyte diversity. This study was undertaken with the objective of isolating endophytes from submerged and aerial part of N. nucifera, study the diversity profile of the isolated endophytes and their antimicrobial, antioxidant, and siderophore production capacity.Methods: Endophytes were isolated from aerial and submerged parts of N. nucifera on different media (Starch Casein Nitrate, Glucose Yeast Extract, Nutrient and Potato Dextrose agar). These were further characterized for morphology (colony characteristics, Gram reaction), physiological characteristics (carbon, nitrogen utilization) and activity (antimicrobial, antioxidant, siderophore production). After dereplication, twelve isolates were studied further.Results: All endophyte isolates were Gram-positive bacteria, and one was a fungus. Isolate L-300 showed the highest antioxidant capacity (238 AAE g FW-1) and L-201 least (10 AAE g FW-1. Antimicrobial activity was exhibited against bacteria and fungal targets, with 50% endophytes active against both bacteria and fungi. Isolates L-003 and L-207 exhibited activity against Gram-negative clinical isolates as also fungi. Siderophore production was shown by 58% isolates with L-208 showing maximum activity.Conclusion: This is the first report on profiling of endophytes from N. nucifera. Results show that aquatic plants harbor diverse microbial population. Many promising isolates (such as L-003, L-211, L-214 and L-300) have been characterized in this study and results obtained of antioxidant, antimicrobial and siderophore production capacity demonstrate further utility in polypharmacological studies for identifying compounds of pharmaceutical and other industrial interest.Â
Authors and Affiliations
Tanuja Ijarwal, Bharti Sharma, Faiza Khan, A. Ibeyaima, Anuj Dwivedi, Narendra Saini, Indira P. Sarethy
Keywords
Related Articles
- EP ID EP577951
- DOI -
- Views 82
- Downloads 0
COMPARISON OF FT-NIR TRANSMISSION AND HPLC FOR GREEN APPROACH TO DETERMINE PARACETAMOL AND ITS DEGRADATION PRODUCT 4-AMINOPHENOL IN PARACETAMOL TABLETS
Objective: Development and validation of Near infrared (NIR) spectroscopic method for determination of paracetamol and its major degradation product 4-aminophenol in paracetamol tablets and show the agreement between the...
DEVELOPMENT AND VALIDATION OF STABILITY INDICATING RP-HPLC METHOD OF NEPAFENAC AND ITS DEGRADATION PRODUCTS: APPLICATION TO DEGRADATION KINETIC
Objective: The objective of present work was to develop and validate simple, precise, accurate and specific stability indicating method for determination of nepafenac in presence of its degradation products and applicati...
EVALUATION OF ANTIDEPRESSANT ACTIVITY OF HERBOMINERAL FORMULATION
Objective: In the search for new therapeutic products for the treatment of neurological disorders, medicinal plant research, worldwide, has constantly progressed, demonstrating the pharmacological effectiveness of differ...
EVALUATION AND OPTIMIZATION OF LEPIDIUM SATIVUM SEED MUCILAGE AS BINDER IN TABLET FORMULATION
Objective: The study elaborates isolation of mucilage from Lepidium sativum seeds and explores it as a tablet binder.Methods: The mucilage from seed was extracted by precipitation of soaked and blended seed in acetone. T...
PROTECTIVE EFFECT OF ETHANOL EXTRACT OF CENTAUREA BEHEN LINN IN CARBON TETRA CHLORIDE-INDUCED HEPATITIS IN RATS
Objectives: To evaluate the hepatoprotective activity of Ethanolic extract of root of Centaurea behen Linn were studied against Wister rats with liver damage induced by carbon tetrachloride (CCl4)Methods: Â The hepatopro...