Enhanced production of doubled haploid lines from parthenogenetic Iranian melon plants obtained of irradiated pollen (Cucumis melo L.)
Journal Title: International Research Journal of Applied and Basic Sciences - Year 2012, Vol 3, Issue 8
Abstract
Haploid and doubled haploid plants are valuable materials for plant breeding purposes such as resistance to many fungal and viral diseases that affect this crop. We produced 48 parthenogenetic melon plantlets via pollination with -irradiated pollen witch 550 Gray of dose cloned them by nodal cuttings, and tested the effects of in vitro and in vivo doubling treatment on survival, ploidy and fruit recovery. Chromosome number was counted in root tip cells from in vitro haploid plants .The most effective procedure for explants recovery was in vitro treatment of shoot tip explants with 500 mg/l colchicine for 12 h in standard medium and plant regeneration from nodal explants treated with 500 mg/L colchicine for 12 h was increased from 33 to 76% by removing apical dominant one week before colchicine treatments.
Authors and Affiliations
Mostafa Nasertorabi| Department of Horticulture, College of Aboureihan, University of Tehran, Tehran, Iran.nasertorabi@ut.ac.ir, Esmaeil Madadkhah| Department of Horticulture, College of Aboureihan, University of Tehran, Tehran, Iran., Hamid Moghbeli| Department of Plant Science, Faculty of Agriculture, University of Jiroft, Jiroft, Iran., Ali Roain| Department of Horticulture. University of Islamic Azad, Jiroft Branch Jiroft, Iran., Ecehagh Moghbeli| Department of Horticulture, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran.
Keywords
Related Articles
- EP ID EP5084
- DOI -
- Views 426
- Downloads 17
The Impact of Play Therapy on Developmental Fears among Preschoolers
The current research is intended to study the impacts of play therapy to on reducing developmental fears among the preschoolers. 46 subjects have been selected through a randomly Cluster Sampling method in a male and fem...
Study of the Status of Educational Philosophy and Determining the Dominant Philosophy from the Point of View of High School Principals of Ardebil City
This research has been carried out with the aim of studying the status of educational philosophy from the principals’ point of view, and the dominant educational philosophy in the high schools of Ardebil city. The method...
Investigating and Identifying the Cybercrimes Relevant to Banking Network and the Quality of Prevention (the case study of Mahabad banks)
Despite the facilities that the application of internet provide, it enables the villains to get aware of the entering ways to public sections’ computer systems and those of secret-based organizations. In addition, the re...
Performance Measurement in the Public Sector Budgeting
This article focuses on the use of performance measurement in public budgeting. The use of performance measurement in budgeting has been advocated at various levels of government. Efforts have been made to incorporate pe...
The relationship between social capital and knowledge sharing in the Ministry of Culture and Islamic Guidance (Iran)
The aim of this study was to investigate the relationship between social capital and knowledge sharing is the Ministry of Culture and Islamic Guidance. They are descriptive correlation study. The study sample consisted o...