Enhanced production of doubled haploid lines from parthenogenetic Iranian melon plants obtained of irradiated pollen (Cucumis melo L.)
Journal Title: International Research Journal of Applied and Basic Sciences - Year 2012, Vol 3, Issue 8
Abstract
Haploid and doubled haploid plants are valuable materials for plant breeding purposes such as resistance to many fungal and viral diseases that affect this crop. We produced 48 parthenogenetic melon plantlets via pollination with -irradiated pollen witch 550 Gray of dose cloned them by nodal cuttings, and tested the effects of in vitro and in vivo doubling treatment on survival, ploidy and fruit recovery. Chromosome number was counted in root tip cells from in vitro haploid plants .The most effective procedure for explants recovery was in vitro treatment of shoot tip explants with 500 mg/l colchicine for 12 h in standard medium and plant regeneration from nodal explants treated with 500 mg/L colchicine for 12 h was increased from 33 to 76% by removing apical dominant one week before colchicine treatments.
Authors and Affiliations
Mostafa Nasertorabi| Department of Horticulture, College of Aboureihan, University of Tehran, Tehran, Iran.nasertorabi@ut.ac.ir, Esmaeil Madadkhah| Department of Horticulture, College of Aboureihan, University of Tehran, Tehran, Iran., Hamid Moghbeli| Department of Plant Science, Faculty of Agriculture, University of Jiroft, Jiroft, Iran., Ali Roain| Department of Horticulture. University of Islamic Azad, Jiroft Branch Jiroft, Iran., Ecehagh Moghbeli| Department of Horticulture, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran.
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