Estimation of Bambuterol in tablet dosage forms by RP-HPLC
Journal Title: Journal of Pharmacy Research - Year 2008, Vol 1, Issue 2
Abstract
A simple, precise, rapid and accurate reverse phase HPLC method was developed for the estimation of Bambuterol in tablet dosage forms. An Inertsil ODS-3CV C18, 250x4.6 mm, coloumn with 5 µm particle size and the mobile phase consisting of 0.1% Trifluoro Acetic acid: Methanol in the ratio of 10:90 v/v was used. The flow rate was 0.8 ml/min and the effluents were monitored at 220 nm. The retention times were 2.381min for bambuterol. The detector response was linear in the concentration of 17.5-420 mcg/ml. The respective linear regression equation being Y= 26705.167x+67644.9694 for Bambueterol. The limit of detection (LOD) is 0.175mcg and 0.35 mcg and the limit of quantification (LOQ) is 0.525mcg for Bambuterol. The percentage assay of Bambuterol was found to be 99.78%. The method was validated by determining its accuracy, precision and system suitability. The results of the study showed that the proposed RP-HPLC method is simple, rapid, precise and accurate, which is useful for the routine determination of Bambuterol in bulk drug and in its pharmaceutical dosage forms.
Authors and Affiliations
V. Kiran Kumar, N. Appala Raju* & Shabana Begum
Keywords
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