EVALUATION OF ANTI-PROLIFERATIVE AND ANTIOXIDANT ACTIVITY OF SWERTIA CHIRATA: IMPLICATIONS IN BREAST CANCER
Journal Title: International Journal of Research and Development in Pharmacy & Life Sciences (IJRDPL) - Year 2016, Vol 6, Issue 1
Abstract
Background: Swertia chirata belonging to Gentianaceae family is a medicinal plant known to exhibit various pharmacological properties. Objective: This study investigates the antiproliferative effects of the aerial parts of S. chirata methanol extract (SCME) against breast cancer cells. Materials and methods: Antiproliferative effects of SCME were assessed on human breast cancer cell lines, MDA-MB-231 and MCF-7 using the MTT cell viability assay at concentrations ranging from 6.25 µg/mL to 400 µg/mL for 24 h, 48 h, and 72 h. Colony formation assay was performed to visualize the growth inhibition. Cellular apoptosis was determined by Hoechst staining. Additionally, enzymatic and non-enzymatic antioxidant activities were also assessed by DPPH, ABTS, TPC, SOD and CAT assays. Results: The results obtained indicate that SCME has potent cytotoxic activity towardsMDA-MB-231 and MCF-7 with anIC50value of 112 ± 4.5 μg/ mL and 180 ± 3 μg/ mL, respectively. Microscopic assessment of cellular and nuclear morphology of SMCE treated breast cancer cells indicated that SCME induced apoptosis as a mode of programmed cell death in these cells. Antioxidant profiling of SCME also indicated a high concentration of polyphenols and presence of antioxidant enzymes SOD and Catalase with potent free radical scavenging activity. Conclusion: Our study demonstrated that SCME has significant antiproliferative activity against human breast cancer cells, MDA-MB-231 and MCF-7 and showed features of apoptotic cell death. Thus, S. chirata can be an enthusiastic target for researchers to exploit its potential as a natural treatment of breast cancer.
Authors and Affiliations
Homa Jilani Khan| Department of Chemistry, Integral University, Lucknow, India, 226 026, Natural Products Research Lab, Department of Biochemistry, King George’s Medical University, Lucknow, India, 226 003, Mohammad Kaleem Ahmad| Natural Products Research Lab, Department of Biochemistry, King George’s Medical University, Lucknow, India, 226 003, Abbas Ali Mahdi| Natural Products Research Lab, Department of Biochemistry, King George’s Medical University, Lucknow, India, 226 003, Namrata Rastogi| Natural Products Research Lab, Department of Biochemistry, King George’s Medical University, Lucknow, India, 226 003, Jamal Akhtar Ansari| Department of Chemistry, Integral University, Lucknow, India, 226 026, Uzma Shahab| Natural Products Research Lab, Department of Biochemistry, King George’s Medical University, Lucknow, India, 226 003, Abdul Rahman Khan| Department of Chemistry, Integral University, Lucknow, India, 226 026
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