Evaluation of Antioxidant Activity And Secondary Metabolites of Menthapiperita L Under Effect of Acetylsalicylic Acid And Methyl Jasmonate
Journal Title: International Research Journal of Applied and Basic Sciences - Year 2014, Vol 8, Issue 3
Abstract
This study investigated the effect of salicylic acid(SA) and methyl jasmonate (MJ) on secondary metabolites as well as antioxidant activities in Menthapiperita L. we used 2 kinds of treatment including (SA) and (MJ) at concentrations of (50, 100 ?m) .Treatments carried out on 7 weeks plants. At first plants were treated with (SA) 3 days then 3 days with (MJ) . In order to measure content of phenolic and flavonoid compound , we used spectrophotometric method. Finally antioxidant activity of the extract was measured in different concentrations using free radical 2, 2- diphenyl -1 PykrylHydrazyl (DPPH). Data Analyzed with SPSS version 16 and one .way ANOVA Experiment was carried out based on completely randomized design with six replication. Results of variance analysis showed that radical scavenging of Menthapiperita Lcultivere significantly decreased by treated (SA,MJ) and reduced antioxidant activities levels compared to control treated with alcohol (P<0/01). Also, treatments (SA,MJ) caused increase in phenolic content. The result of this treatment showed these treatments stimulated amount of essential oil, total phenolic content but it dosent have positive effect on flavonoids of MenthapiperitaL.
Authors and Affiliations
Azita Shabrangi *| Islamic AzadUniversity , Pharmaceutical Sciences Branch Tehran, Iran, email: a.shabrangi@tmu.ac.ir, Leyla Mehrabi| Islamic AzadUniversity , Pharmaceutical Sciences Branch Tehran, Iran
Keywords
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