Evaluation of cytochrome concentration in peripheral blood Iymphocytes of patients with Iaryngeal cancer
Journal Title: Otolaryngologia Polska - Year 2011, Vol 65, Issue 6
Abstract
SUMMARY The aim of the study was evaluation of cytochrome concentration in the peripheral blood lymphocytes of patients with laryngeal cancer. The study was conducted in a group of 62 patients presenting different clinical advancement of the disease. The study material consisted of the studied population's peripheral blood from which T Iymphocytes were isolated and incubated with monoclonal antibodies. To evaluate antigens' expression, a FACS Galibur flow cytometre was used; the evaluated cells were labeled with fluorochrome-conjugated monoclonal antibodies. Next, mononuclear cells were rinsed with cold PBS and suspended in the lysis buffer. In the obtained celt lysate the c cytochrome concentration was determined with the use of an immunoenzymatic test, HumanCytochrome cELISA Kit (BenderMed Systems, Austria). Obtained results were compared with the measurements taken in 20 healthy individuals who constituted the control group. On the basis of conducted study it was found that the level of c cytochrome concentration was significantly increased in the T CD3+ Iymphocytes in the peripheral blood of patients with stage IV of Iaryngeal cancer. A positive correlatio was also found between the cytochrome level in lymphocytes and the advancing stage of the disease. Our own observation give grounds to conjecture that together with the progress of Iaryngeal cancer the energetic potential of Iymphocytes increases and so does the readiness of the lymphatic cells to undergo the redox processes, therefore the amount of c cytochrome in the cells increases.
Authors and Affiliations
Elżbieta Hryciuk-Umer, Krzysztof Kupisz, Agnieszka Bojarska-Junak, Adrian Andrzejczak, Eliza Trzaskowska, Krystian Kotiuszko, Janusz Klatka
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