Evaluation of different potato somaclones for resistance to Alternaria solani Sorauer, through the use of artificial inoculation under field conditions
Journal Title: Biotecnología Vegetal - Year 2001, Vol 1, Issue 2
Abstract
The use of plant pathogens, for the genetic improving programs is an important matter to guarantee its success. Utilization of phytopathogenic isolates from natural source, let us to employ rapid screening in some important genotypes. In this work it was used a mycelium homogenizated of Alternaria solani Sor (strain IBP-As-4), with a relative concentration of 1.7x10 5 ufc ml -1 . The inoculum was applied by spraying to the somaclons (obtained by mutagenic and in vitro selection with culture filtrated of the fungus), also to the susceptible (Desireé) and resistant (Solanum chacoense) cultivars, respectively. The first symptoms appeared after seventh day of inoculation. The behavior between somaclons and testers in the final evaluation were different. Solanum chacoense was the most resistant genotype, the somaclons IBP-93 and IBP-38 were more susceptible than Desireé. The somaclons IBP- 27 reached a similar affectation score respect to Solanum chacoense. The rest of the somaclons had an intermediate performance. It was proved the important of using of pathogenic strains of Alternaria solani Sor, for making selection where the natural inoculum source it is weak.
Authors and Affiliations
Michel Leiva-Mora, Novisel Veitía, Miguel Angel Dita Rodríguez
Keywords
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