Evaluation of different potato somaclones for resistance to Alternaria solani Sorauer, through the use of artificial inoculation under field conditions
Journal Title: Biotecnología Vegetal - Year 2001, Vol 1, Issue 2
Abstract
The use of plant pathogens, for the genetic improving programs is an important matter to guarantee its success. Utilization of phytopathogenic isolates from natural source, let us to employ rapid screening in some important genotypes. In this work it was used a mycelium homogenizated of Alternaria solani Sor (strain IBP-As-4), with a relative concentration of 1.7x10 5 ufc ml -1 . The inoculum was applied by spraying to the somaclons (obtained by mutagenic and in vitro selection with culture filtrated of the fungus), also to the susceptible (Desireé) and resistant (Solanum chacoense) cultivars, respectively. The first symptoms appeared after seventh day of inoculation. The behavior between somaclons and testers in the final evaluation were different. Solanum chacoense was the most resistant genotype, the somaclons IBP-93 and IBP-38 were more susceptible than Desireé. The somaclons IBP- 27 reached a similar affectation score respect to Solanum chacoense. The rest of the somaclons had an intermediate performance. It was proved the important of using of pathogenic strains of Alternaria solani Sor, for making selection where the natural inoculum source it is weak.
Authors and Affiliations
Michel Leiva-Mora, Novisel Veitía, Miguel Angel Dita Rodríguez
Keywords
Related Articles
- EP ID EP697715
- DOI -
- Views 49
- Downloads 0
Use of Gibberellins and foliar fertilization during acclimatization of Vitroplants of Pineapple Cayenne Lisa c.v. ̈Serrana ̈
In the last few years the productions levels of pineapple have decreased. The lack of planting material is one of the factors responsible for such a decrement. Two protocols for pineapple micropropagation have been recen...
In vitro establishment of apex and nodule segments of Swietenia macrophylla King
The application of tissue culture techniques in woody plants offers a valuable alternative for the propagation of elite trees. With the objective of obtaining the in vitro establishment of mahogany (Swietenia macrophyll...
Protocol for Anthurium cubense Engler micropropagation from botanic seed
The popularity of Anthurium cubense, like pot plant, every day it is increased. Likewise, it has begun to extend the line of cultivated products for their employment as foliage. However, the traditional propagation of...
Effect of the combined use of two cytokinins in the multiplication and regeneration of adventitious banana buds cv. ‘Gros Michel’ (Musa AAA)
In breeding programs it is important to have an efficient plants regeneration system. In the cultivar ‘Gros Michel’ (Musa AAA) it is difficult to obtain adventitious buds due to high apical dominance. The present investi...
Minitubers production of four potato Cuban varieties in greenhouse with zeolite as substrate
The obtaining of original seed of national potato varieties (Solanum tuberosum L.) is of great importance. The objective of the present work was to obtain minitubers from four national potato varieties in a greenhouse w...