Evaluation of different potato somaclones for resistance to Alternaria solani Sorauer, through the use of artificial inoculation under field conditions
Journal Title: Biotecnología Vegetal - Year 2001, Vol 1, Issue 2
Abstract
The use of plant pathogens, for the genetic improving programs is an important matter to guarantee its success. Utilization of phytopathogenic isolates from natural source, let us to employ rapid screening in some important genotypes. In this work it was used a mycelium homogenizated of Alternaria solani Sor (strain IBP-As-4), with a relative concentration of 1.7x10 5 ufc ml -1 . The inoculum was applied by spraying to the somaclons (obtained by mutagenic and in vitro selection with culture filtrated of the fungus), also to the susceptible (Desireé) and resistant (Solanum chacoense) cultivars, respectively. The first symptoms appeared after seventh day of inoculation. The behavior between somaclons and testers in the final evaluation were different. Solanum chacoense was the most resistant genotype, the somaclons IBP-93 and IBP-38 were more susceptible than Desireé. The somaclons IBP- 27 reached a similar affectation score respect to Solanum chacoense. The rest of the somaclons had an intermediate performance. It was proved the important of using of pathogenic strains of Alternaria solani Sor, for making selection where the natural inoculum source it is weak.
Authors and Affiliations
Michel Leiva-Mora, Novisel Veitía, Miguel Angel Dita Rodríguez
Keywords
Related Articles
- EP ID EP697715
- DOI -
- Views 58
- Downloads 0
Morpho-agronomic and organoleptic response of five banana cultivars (Musa spp.) under field conditions
In Cuba, banana production is destined for the national market as fresh fruit and plays an essential role in food security. The objective of this work was to evaluate in field conditions the morpho-agronomic and organole...
Obtaining somatic embryos and establishing embryogenic cell suspensions in the banana cultivar 'Navolean' (AAB)
Cells suspension of plantains and bananas with promising results have been reported internationally, however, in Cuba it is not at so for AAB group. So, the following working objectives have to be considered: in vitro mu...
Protocol for isolation of Moniliophthora roreri (Cif and Par) Evans et al. from cacao fruits cv. ‘National’ in the Ecuadorian Amazonia
Moniliophthora roreri is a pathogen of cocoa (Theobroma cacao L.) that causes high economic losses in Ecuador. This paper is intended to present a protocol for the isolation of the pathogen from cocoa fruits cv. ‘Natio...
In vitro establishment of Heliconia rostrata Ruiz & Pavón and Heliconia wagnerina Petersen
The heliconia have wide appeal as ornamental plants and their environmental significance. The species Heliconia rostrata and H. wagneriana are planted in the Botanical Garden of Cienfuegos, Cuba. The aim of this study...
Phytochemical characterization of the threatened specie Fraxinus caroliniana Mill subsp. cubensis (Griseb.) Borhidi
Fraxinus caroliniana Mill subsp. cubensis (Griseb.) Borhidi is commonly known as buffalo, represents an endemic subspecies and categorized as critical danger of extinction in Cuba. This work aimed to characterize the p...