EVALUATION OF GLYCOLIPIDS OF SOME EGYPTIAN MARINE ALGAE AS A SOURCE OF BIOACTIVE SUBSTANCES
Journal Title: International Research Journal of Pharmacy (IRJP) - Year 2011, Vol 2, Issue 3
Abstract
Glycolipids in five species of marine algae: two species of Rhodophyta (Laurencia popillose, Galaxoura cylindriea); and one species of Chlorophyta (Ulva fasciata), and two species of Phaeophyta (Dilophys fasciola, Taonia atomaria ) collected from Red and Mediterranean sea, respectively were extracted, purified on silica gel column chromatography and identified by liquid chromatography MS/MS. Total glycolipid contents (GL) (as % of total lipid) were found to be in ranges 10.9 to 28.7%. T. atomaria had the highest level (28.7%) followed by L. popillose (22.5 %). GL groups were analyzed for their sugars and fatty acids composition, and the result showed that the highest carbohydrate content of GLs were found in U. fasciata (6.05%) and L. popillose (5.8%), and characterized by high content of monosaccharide: mannouronic acid, galactose and rhamnose. Amongst of the glycolipids of algal species, the most predominate fatty acid identified by GC were palmatic (C16:0 19.20 - 65.89% of total fatty acid), ecosatrienoic (C20:3 7.52 - 54.41%). GL analyzed by LC/MS/MS, revealed the peak at m/z 956 corresponding to the molecular formula of C51H104O17 was the most abundant molecular ion among all GLs of algal species and its fragments peaks at m/z 617(C37H58O4) and m/z 337 (C21H58O3), were tentatively identified as digalactosyldiacylglycerol (DGDG). The in vitro anticancer, antimicrobial, and antiviral activities of algal glycolipids were evaluated. GL of all algae species showed a remarkable antiviral activity in dose dependent manner. GL from D. fasciola has shown the most potent effect against HSV1 (IC50 of 10 µg/ml), comparable to that of the current antiviral drug acyclovir (IC50 55 µg/ml). On the other hand, GL of all algal species possessed a moderate antimicrobial activity. GL of T. atomaria exhibited a high inhibition effects against all test microorganisms, with MIC value ranged from 60 to 80 µg/ml. Moreover, all algal GL exhibited remarkable anticancer activities against both breast (MCF7) and liver human (HepG2) cancer cells, with an IC50 values ranging from 0.47 to 2.89 µg/ml.
Authors and Affiliations
Gamal El Baroty , El-Baz , Farouk K , Abd-Elmoein Ibtisam, Abd El Hanaa H, Mohamed Ali , Eman Ibrahim
Keywords
Related Articles
- EP ID EP145452
- DOI -
- Views 119
- Downloads 0
REVELATION OF MECHANISM OF ACTION OF RHIZOPHORA MUCRONATA POIR. BARK EXTRACT FOR ITS ANTIDIABETIC ACTIVITY BY GUT PERFUSION AND SIX SEGMENT METHOD IN LONG EVANS RATS
The aqueous bark extract of Rhizophora mucronata Poir. is used in Bangladesh as a hypoglycemic aid without any knowledge about its probable mode of action . It is an effort to assess the claimed hypoglycemic property of...
DIFFERENTIAL DIAGNOSIS OF LONG TERM TONGUE ULCERS
Oral ulcers is a very common disorder of the oral mucosa. Patients with signs or symptoms of oral ulcers are sometimes referred to gastroenterology clinics, however, in most instances the ulcers does not reflect gastroin...
SYNTHESIS OF SOME SUBSTITUTED BENZIMIDAZOLE DERIVATIVES FOR ANTIBACTERIAL ACTIVITY
In the present study synthesis and evaluation of antibacterial activity of various substituted benzimidazole derivatives condensed with different amines was undertaken. The derivatives were synthesized in moderate to goo...
DESIGN DEVELOPMENT AND EVALUATION OF MODIFIED RELEASE TABLET OF MONTELUKAST SODIUM USING ETHYL CELLULOSE AND TRAGACANTH
The purpose of this research was to prepare a modified release tablet of montelukast sodium. Montelukast sodium is Leukotriene antagonist which is rapidly absorbed after the oral administration. The drug was mixed with E...
ACTIVE PRINCIPLES AND MEDIAN LETHAL DOSE OF CURCUMA LONGA LINN.
The present study aimed to determine the active principles and median lethal dose (LD50) of Curcuma longa (Haldi) by conducting phytochemical and toxicity (acute and chronic) studies. The hydroalcoholic extract (HAE) of...