Evaluation of In-Vitro Antioxidant Activity in Ficus religiosa (L.) Leaves
Journal Title: International Journal of Research in Pharmacy and Science - Year 2011, Vol 1, Issue 2
Abstract
Medicinal plants have been used in traditional medicine for the treatment of several diseases. In India, medicines based on herbal origin have been the basis of treatment and cure for various diseases and physiological abnormalities under practice such as Ayurveda, Siddha and Unani. Research in herbal medicine has recently been revolutionized with the identification of several botanical plants with established physiological effect and efficacy for clinical condition either alone or combination with pharmaceuticals. Ayurveda is a comprehensive natural health care system that organized in India more than 5000 year ago. Plant antioxidants are composed of a broad variety of different substances like ascorbic acid and tocopherols, polyphenolic compounds, or terpenoids. They perform several important functions in plants and humans. In this study, antioxidant activity of ethanolic extract of Ficus religiosa Linn. (EEFR) leaf was investigated for its free radical scavenging activity by adopting various in vitro models. The extract was investigated for its antioxidant activity by 1,1--diphenyl, 2-picryl hydrazyl (DPPH) radical scavenging activity, hydroxyl radical scavenging activity, reducing capacity, hydrogen peroxide activity, determination of total phenolic content using Folin-Ciocalteu's phenolic reagent. EEFR showed maximum scavenging of DDPH radical 91.20% at 250 µg/ml concentration and hydrogen peroxide and reducing power were also dose dependent. The IC50 values were found to be 71.10µg/ml and 22.5 µg/ml of EEFR and ascorbic acid respectively. The total phenolic content evaluated that 1 mg of extract contained 3.2µg Gallic acid equivalents of phenols respectively. The extract showed significant results when compared with standard groups.
Authors and Affiliations
Hement Kumar, Sanjay Yadav, V Rao
Keywords
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