Evaluation of PCR Assay Using Specific Primers in Diagnosis of Canine Ehrlichiosis and Babesiosis:A Study on Herd and Stray Dogs in Shiraz
Journal Title: Journal of Alternative Veterinary Medicine - Year 2017, Vol 1, Issue 1
Abstract
Canine tick-borne diseases are regarded as emerging problems within Iran and throughout the world. The present article aims at investigating Ehrlichia canis and Babesia canis infections in herd and stray dogs in Iran. The present study is therefore an attempt to compare PCR assay with specific primers in order to detect the organisms at the species level. This study is based on PCR amplification of the 16S rRNA, 18S rRNA and p28 genes. Concerning Babesia canis, species-specific primers BAB3 and BAB1 are compared with genus-specific primers B-GA1B and 16S8FE. Moreover, for Ehrlichia canis, species-specific primers ECp28-F and ECp28-R are compared with genus-specific primers RLB - F2 and RLB - R2. For the purpose of this study, 280 herd and stray dogs from seven different regions in south of Iran were monitored. As the results revealed, molecular surveillance of tick-borne diseases was based on PCR amplification of the 16S rRNA, 18S rRNA and p28 genes. For Ehrlichia canis, PCR was positive with genus-specific primers (B-GA1B and 16S8FE ) and species-specific primers (ECp28-F and ECp28-R),1.4% (4 of 280) of blood samples. For Babesia canis, PCR was positive with genus-specific primers (RLB - F2 and RLB - R2), 1% (3 of 280) and species-specific primers for Babesia canis (BAB1 and BAB3) 0.4% (1 of 280) of blood samples, respectively. Sequencing results showed that one herd dog was infected with Babesia canis, four herd dogs were infected with Ehrlichia canis, and one herd dog showed co-infection with Ehrlichia canis and Babesia canis. This has been the first report of the co-infections of both Ehrlichia canis and Babesia canis in naturally infected dogs in South of Iran.
Authors and Affiliations
Masih Bigdeli, Mohammad Mehdi Namavari
Keywords
Related Articles
- EP ID EP711239
- DOI -
- Views 37
- Downloads 0
Inhibitory Effect of Hydroalcoholic Extract of Mint Plant on Escherichia ColiIsolated fromLambs and Goat Kidswith Diarrheain Kazeroun
In spite of advances in the management and prevention techniques as well as strategies for diarrhea treatment, it is still a very common and costly disease affecting the small ruminants. The present study aimed at determ...
The Protective Effects of Alcoholic Ginger Extract on Histological Changes of Testis in Doxorubicin-Treated Wistar Rats
Doxorubicin (DOX) has a wide spread use in cancer chemotherapy and Zingiber officinale (ginger) has androgenic property and effects on fertility. The aim of the present study was to investigate the protective effects of...
The Effect of Ginger on the Growth of Lactobacillus Acidophilus and Bifidobacterium Bifidum in Probiotic Dairy Products
Probiotics are regarded as live microorganisms that provide health benefits when consumed. In the present study, the potentiality of producing foods containing probiotic bacteria with milk, yoghurt and ginger bases was e...
The Effect of Tarragon on the Growth of Lactobacillus casei and Lactobacillus paracasei in Probiotic Milk
Probiotics are live microbial food supplements which benefit the health of consumer by maintaining or improving their intestinal microbial balance. This study aimed at investigating the effect of different doses of tarra...
An Investigation into the Effects of Aqueous Extract of Carum Copticum on Renal Tissue and Uric Acid in Male Wistar Rats
Of the nine chemical compounds identified in the Carum Copticum extract, thymol, γ-terpinen, and parasaymn are considered as the main compounds utilized as antiemetic, anti-asthma, anti-nausea, and spasmodic, locally use...