Evaluation of Phytochemical and Antioxidative properties of Rosa kordesii Petal Extracts
Journal Title: IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS) - Year 2016, Vol 11, Issue 3
Abstract
Plant extracts have been reported to have contributed to the increase of nutritional values which are important to the human health. Investigations were carried out to evaluate the phytochemicals content and antioxidant activity of Rosa kordesii petal extracts in different solvents. Methanol, water and n-hexane were used as solvent. Under an observational analytic study revealed the presences of alkaloids, flavonoids, phenols, carbohydrate, resins, saponins, steroids, tannins, anthracenosides and coumerins as phytochemicals in the extracts. The order of phenolic contents was as follows: aqueous extract; 461.93±3.24, methanolic extract; 349.59±3.62; and n-hexane extract; 42.42±1.71mg GAE/g residue whereas total flavonoid contents were found to be 78.11±2.00, 168.22±2.29 and 45±2.00 mg of catechin/g of extract, respectively. Antioxidant activity of each extract was carried out by total antioxidant capacity test, DPPH, ABTS and ferric reducing antioxidant power (FRAP) assay by using ascorbic acid as standard. Methanolic extract was found to be the most effective in all assays, except total antioxidant capacity test with IC50 of 21.93μg/ml (DPPH Assay) and 10.38μg/ml (ABTS Assay). The antioxidant activities were positively associated with the total phenolic and flavonoid contents of the extracts. Further study on different solvent extracts of Rosa kordesii would be carried out to elucidate the active principles for its outmost activity.
Authors and Affiliations
Belal MH1, Yesmin MR, Islam MD3, Mamun MA, Hasan N5, Rahman MA6, Tasnin MN, Ara T9, Rabbi MAR, , Rahman MS, Khan MMH, Karim MR
Keywords
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