Evaluation on acute toxicity of tetrabuthylammonium bromide ionic liquid at histological structure of some organs in zebrafish (Danio rerio)
Journal Title: Aquaculture, Aquarium, Conservation & Legislation - International Journal of the Bioflux Society - Year 2010, Vol 3, Issue 5
Abstract
In this work, we evaluated the acute toxicity caused by zebrafish (Danio rerio) exposure to various concentrations of tetrabuthylammonium bromide. The ionic liquid’s acute toxicity on zebrafish was assessed according to the lethal effects after a 96-hour exposure. To perform the test on tetrabuthylammonium bromide’s acute toxicity, we carried out three series of experiments; during each series, we used five different ionic liquid concentrations, respectively: 10; 25; 50; 75; 100 mg L-1 for the experiment I, 200; 400; 600; 800; 1000 mg L-1 for the experiment II and 1500; 2000; 3000, 4000 and 5000 mg L-1 for the experiment III. All these batches were compared with the control variant. The histopathological examination was performed on individuals from the control group and on all the dead fish. The results of the acute toxicity test reveal that the LC50 value after 96 hours is between 2500 and 3000 mg L-1. The fish exposed to 4000, respectively 5000 mg L-1 tetranuthylammonium bromide died during the first 24 hours after exposure. The values obtained for tetrabuthylammonium bromide are much smaller than the data obtained for LC50 at 96 hours for other solvents like acetone (30642 mg L- 1), respectively DMF (12220 mg L-1), but much bigger than the toluene (60 – 313 mg L-1), benzene (203mg L-1), phenol (5 mg L-1), etc. The histopathological study on gill, skin, liver, kidney and intestine reveal that the changes occurred within the structure of these organs are intensified with the increase of tetrabuthylammonium bromide concentration.
Authors and Affiliations
Gabi Dumitrescu, Liliana Petculescu-Ciochină, Ioan Bencsik, Dorel Dronca
Keywords
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