EXOPOLYSACCHARIDES PRODUCTION BY LACTIC ACID BACTERIA STRAINS ISOLATED FROM FERNMENTED PRODUCTS
Journal Title: Мікробіологія і біотехнологія - Year 2017, Vol 0, Issue 4
Abstract
Aim. To search for lactic acid bacteria (LAB) strains that produce exopolysaccharides among the isolates from traditional dairy products, fermented fruits and vegetables. Methods. Identification of the isolates were carried out according to morphological-cultural and physiology-biochemical properties. The ability to produce and amount of exopolysaccharides were determined on MRS mediua with 5% carbohydrate (sucrose, glucose, fructose or lactose). Results. 25% LAB strains were able to produce EPS on solid media with different carbon sources. All Leuconostoc spp. produced EPS on media with sucrose (in amount 0,40–18,00 g/l) and 93% of Pediococcus spp. strains (0,35–9,40 g/l), whereas among Lactobacillus spp.strains – only 37,5% of strains (0,15–2,75 g/l). At the same time, Lactobacillus spp.strains produced EPS on media with glucose, fructose or lactose in amount 0,15–0,90 g/l. Conclusions. The ability of LAB strains to produce EPS depended on genus affiliation and available carbohydrate in the medium. The LAB strains promising for further studies of biological activity and the structure of their exopolysaccharides have been selected.
Authors and Affiliations
I. L. Garmasheva, N. K. Kovalenko, O. M. Vasyluk, L. T. Oleschenko
Keywords
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- EP ID EP316207
- DOI 10.18524/2307-4663.2017.4(40).118933
- Views 115
- Downloads 0
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