MYCOLYTIC ACTIVITY OF PHOSPHATE MOBILIZING SOILBA CTERIA OF GENUS BACILLUS COHN
Journal Title: Мікробіологія і біотехнологія - Year 2015, Vol 0, Issue 1
Abstract
The aim of this research was study of the chitinolytic activity of phosphateobilizing soil bacteria of genus Bacillus and their antagonistic properties towards microscopic fungi. Methods. In this research 16 strains of phosphate-mobilizing soil bacteria of genus Bacillus and saprophytic and plant pathogenic strains of micrtomycetes were investigated. The antagonistic activity of bacilli against micromycetes was determined by the method of blocks and holes. Bacilli on agar medium and also their supernatants were used. Inhibition of fungal growth under co-cultivation with bacilli under static conditions in the liquid culture medium was determined gravimetrically by the difference of dry weight mycelium in control and in experiment. Bacilli chitinolytic activity was determined photocolorimetrically by the formation of reducing sugars by hydrolysis of colloidal chitin using 3,5-dinitrosalicylic acid. Student test for 5% significance level was used for statistical processing of the obtained data. Results. The presence of chitinolytic activity in all tested bacilli in colloidal chitin medium was established. B. megaterium 16, B. pumilus 7, B. cereus v mycoides 10 strains showed high chitinolytic activity, they were also characterized by high antagonistic activity against plant pathogenic fungi (Fusarium oxysporum 54 and Trichothecium roseum 658). Bacilli were capable to using autoclaved mycelium as the sole of carbon and nitrogen source. Cultivation of bacteria and microscopic fungi under static conditions showed growth inhibition and changes in morphological features – blistering and areas of lysis of mycelium. Conclusions. It was established that phosphate-mobilizing strains of soil bacteria of the genus Bacillus showed chitinolytic activity and growth inhibition of plant pathogens and caused their morphological changes in co-culture. The direct relationship between the antagonistic and chitinolytic activity of bacilli was demonstrated. The bacilli ability to use autoclaved fungal mycelium as a sole source of carbon and nitrogen was showed on the example of B. cereus v. mycoides 10.
Authors and Affiliations
А. А. Roy, E. S. Kharkevich
Keywords
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- EP ID EP376560
- DOI 10.18524/2307-4663.2015.1(29).48035
- Views 93
- Downloads 0
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