RFLP-ANALYSIS OF BRADYRHIZOBIUM JAPONICUM NODULE BACTERIA, WIDESPREAD IN AGROCENOSIS OF UKRAINE
Journal Title: Мікробіологія і біотехнологія - Year 2017, Vol 0, Issue 4
Abstract
Aim. To investigate the genetic diversity of soybean nodule bacteria, widespread in agrocenoses of Ukraine. Methods. Nodule bacteria were isolated from soybean nodules grown in soil samples selected in different regions of Ukraine. The amplification of the intergenic region 16S-23S rRNA (ITS-region) was conducted with the use of primers FGPS1490-72 and FGPL132-38. For PCR-products restriction there were applied restriction endonucleases MspI, HaeIII and NdeII. Results. The diversity of Bradyrhizobium japonicum strains with different growthrates was investigated using PCR-RFLP method. According to analysis of 16S-23S rDNA with enzymes MspI, HaeIII and NdeII it was obtained that all the intensivegrowing strains had the same restriction profiles, so they were combined into a single genetic group. Based on ITS-region restriction the slow-growing strains were assigned into two genetic groups using MspI enzyme, three genetic groups using HaeIII and four genomogroups using NdeII enzyme. Сonclusions. High heterogeneity of B. japonicum strains allocated from soybean agrocenoses was found. They belong to different ITS-types according to the structure of intergenic region. Slow-growing strains used to be more heterogeneous, in comparison with more intensive-growth strains, which formed a homogeneous genomogroup.
Authors and Affiliations
D. V. Krutylo
Keywords
Related Articles
- EP ID EP316163
- DOI 10.18524/2307-4663.2017.4(40).111872
- Views 119
- Downloads 0
QUALITY OF JUICE AND WINE MADE OF GRAPE VARIETIES AROMATNY AND CABERNET SAUVIGNON AFTER TREATMENT OF VINES BY BIOPREATION AGROMAR
Aim. The purpose of this work was to find out the effect of processing of grape plants by the biopreparation AgroMar on the quality of wine materials and wine of varieties Aromatny and Cabernet Sauvignon. Methods. The bi...
EFFECT OF ANTIBIOTICOTHERAPY ON THE TOTAL AMOUNT OF OXALATE-DEGREADING BACTERIA IN THE RATS INTESTINAL TRACT
Aim. To determine the quantity of oxalate-degrading bacteria (ODB) in fecal biopsy and mucosa-associated microbiota of the colon and small intestine of rats. Methods. Study was conducted on male Wistar rats (weight 170–2...
ADENOVIRUS INFECTION AS ONE OF POSSIBLE CAUSES OF FATTY HEPATOSIS IN ONE DAY–OLD CHICKENS
Aim. To investigate the role of adenovirus infection in the development of fatty liver disease in one day-old chicks. Methods. The condition of the internal organs of poultry assessed by autopsy, the amount of vitamin A...
MONITORING OF YEASTS CAUSED BAKERY PRODUCTS CONTAMINATION
Aim. The aim of the research was to determine the contaminants of bread, caused lime disease. Methods. Microbiological methods were used to determine the yeast contamination of bakery products with signs of bread’s disea...
OPTIMIZATION OF THE NUTRIENT MEDIUM FOR CULTIVATION OF PSEUDOMONAS MALTOPHILIA ONU329 STRAIN – SORBENT OF HEAVY METALS IONS AND DESTRUCTOR OF OIL HYDROCARBONS
Aim. Optimization of the nutrient medium (NM) for cultivation of Pseudomonas maltophilia ONU-329 strain – sorbents of heavy metals ions and destructors of oil hydrocarbons to obtain the maximum amount of biomass of bacte...