ISOLATION OF CU (II) FROM AQUEOUS SOLUTIONS BY IMMOBILIZED BACTERIAL CELLS OF THE GENUS PSEUDOMONAS

Journal Title: Мікробіологія і біотехнологія - Year 2017, Vol 0, Issue 3

Abstract

Aim. To develop a method for purifying water from Cu (II) using bacteria of the genus Pseudomonas immobilized on natural sorbents. Methods. For research use nonpathogenic bacterial strains P. cepacia ONU327, P. fluorescens ONU328, P. maltophilia ONU329. The content of Cu (II) in water was determined by atomic absorption method for flame atomic absorption spectrophotometer "Saturn" in the flame of a mixture of "air - propane - butan" at the wavelength for Cu (II) 324,7 nm statistical processing results of research conducted by using the computer program "Microsoft Office Excel 2003" with the definition of Student's t-test. Results. It was experimentally established that the water purification from Cu (II) at a concentration of 50 mg/dm3 in the presence of the association of bacteria P. fluorescens ONU328, P. maltophilia ONU329, P. cepacia ONU327 immobilized on bentonite clay and chitosan reduced the concentration of copper ions by 70– 75%, which corresponded to a residual copper concentration of 15.0–12.5 mg/ dm3 . After deep purification of water from Cu (II) by immobilized bacteria in the composition of the biofloculs (in the presence of hydrogen peroxide and calcium chloride, the residual content of Cu (II) was 0.10 ± 0.008 mg/dm3 , did not exceed the maximum allowable concentration in water of the sanitary-household water use (0.1 mg/ dm3 ) and in water for discharge into the sewage system. Conclusion. The developed method for deep water purification from Cu (II) using bacteria of the genus Pseudomonas immobilized on natural sorbents (clay and chitosan) and in the composition of biofloculs allows to reduce the content of copper ions from 50 mg/dm3 to 0.10±0.008 mg/dm3 and discharge clean water into the sewage system.

Authors and Affiliations

О. G. Gorshkova, Т. V. Gudzenko, О. V. Voliuvach, Т. О. Beliaeva, І. Р. Konup

Keywords

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  • EP ID EP316306
  • DOI 10.18524/2307-4663.2017.3(39).110878
  • Views 94
  • Downloads 0

How To Cite

О. G. Gorshkova, Т. V. Gudzenko, О. V. Voliuvach, Т. О. Beliaeva, І. Р. Konup (2017). ISOLATION OF CU (II) FROM AQUEOUS SOLUTIONS BY IMMOBILIZED BACTERIAL CELLS OF THE GENUS PSEUDOMONAS. Мікробіологія і біотехнологія, 0(3), 66-74. https://europub.co.uk/articles/-A-316306