ISOLATION OF CU (II) FROM AQUEOUS SOLUTIONS BY IMMOBILIZED BACTERIAL CELLS OF THE GENUS PSEUDOMONAS
Journal Title: Мікробіологія і біотехнологія - Year 2017, Vol 0, Issue 3
Abstract
Aim. To develop a method for purifying water from Cu (II) using bacteria of the genus Pseudomonas immobilized on natural sorbents. Methods. For research use nonpathogenic bacterial strains P. cepacia ONU327, P. fluorescens ONU328, P. maltophilia ONU329. The content of Cu (II) in water was determined by atomic absorption method for flame atomic absorption spectrophotometer "Saturn" in the flame of a mixture of "air - propane - butan" at the wavelength for Cu (II) 324,7 nm statistical processing results of research conducted by using the computer program "Microsoft Office Excel 2003" with the definition of Student's t-test. Results. It was experimentally established that the water purification from Cu (II) at a concentration of 50 mg/dm3 in the presence of the association of bacteria P. fluorescens ONU328, P. maltophilia ONU329, P. cepacia ONU327 immobilized on bentonite clay and chitosan reduced the concentration of copper ions by 70– 75%, which corresponded to a residual copper concentration of 15.0–12.5 mg/ dm3 . After deep purification of water from Cu (II) by immobilized bacteria in the composition of the biofloculs (in the presence of hydrogen peroxide and calcium chloride, the residual content of Cu (II) was 0.10 ± 0.008 mg/dm3 , did not exceed the maximum allowable concentration in water of the sanitary-household water use (0.1 mg/ dm3 ) and in water for discharge into the sewage system. Conclusion. The developed method for deep water purification from Cu (II) using bacteria of the genus Pseudomonas immobilized on natural sorbents (clay and chitosan) and in the composition of biofloculs allows to reduce the content of copper ions from 50 mg/dm3 to 0.10±0.008 mg/dm3 and discharge clean water into the sewage system.
Authors and Affiliations
О. G. Gorshkova, Т. V. Gudzenko, О. V. Voliuvach, Т. О. Beliaeva, І. Р. Konup
EXOPOLYSACCHARIDES PRODUCTION BY LACTIC ACID BACTERIA STRAINS ISOLATED FROM FERNMENTED PRODUCTS
Aim. To search for lactic acid bacteria (LAB) strains that produce exopolysaccharides among the isolates from traditional dairy products, fermented fruits and vegetables. Methods. Identification of the isolates were carr...
FATTY ACID COMPOSITION OF BACTERIA OF ENTEROCOCCUS ITALICUS ONU547 STRAIN
Aim. Determination of fatty acid composition of bacteria of bacteriocin producing the strain E. italicus ONU547 and of its preliminary identification. Methods. The strain E. italicus ONU547, isolated from Thai fermented...
TRITICUM DICOCCUM (SCHRANK) SCHUEBL SENSITIVITY TO ANDROGENESIS IN VITRO
Aim. To estimate the influence of cultural media on induction and plant regeneration of emmer. Methods. The research was performed on two spring emmer genotypes. Plants were cultivated in field conditions. Spike pretreat...
NO-SYNTHASE ACTIVITY OF PERIPHERAL BLOOD LEUKOCYTES IN PATIENTS WITH ACNE VULGARIS
The aim is to study changes in the activity of distinct NO-synthase isoforms of blood lymphocytes in persons with acne vulgaris, for those individuals with disease accompanied by seeding of film-forming and planktonic st...
ANTAGONISTIC ACTIVITY OF LACTOBACILLUS PLANTARUM, ISOLATED FROM PLANT SOURCES OF UKRAINE AND FRANCE, AGAINST PHYTOPATHOGENIC BACTERIA
Aim. Study of antagonistic activity of L. plantarum isolates from different geographical regions against phytopathogenic bacteria and selection of the most active isolates for development of biological preparations for p...