FATTY ACID COMPOSITION OF BACTERIA OF ENTEROCOCCUS ITALICUS ONU547 STRAIN
Journal Title: Мікробіологія і біотехнологія - Year 2018, Vol 0, Issue 3
Abstract
Aim. Determination of fatty acid composition of bacteria of bacteriocin producing the strain E. italicus ONU547 and of its preliminary identification. Methods. The strain E. italicus ONU547, isolated from Thai fermented cabbage was used in the work. The bacteria were cultivated on the medium de Man, Rogosa and Sharpe (MRS) with 1.5% agar. The fatty acid composition of bacteria of E. italicus ONU547 strain was determined by gas chromatograph Agilent 7890 equipped with Ultra 2 column (25 m х 0,2 mm) (Agilent Technologies, Santa Clara, California, USA) using the automatic system of identification of microorganisms MIDI Sherlock. Results. Octadecenoic acids are dominating fatty acids (61.52%) of bacteria of E. italicus ONU547 strain and it has been showed for the first time. Hexadecanoic acid is also present at relatively high concentration and comprised 26.41% from total amount. Other detected fatty acids (С17:0, С20:1w7c, С12:0, С19:0 cyclo w8c, С18:0, С14:0, С16:1) are present in much lower quantities and comprised from 0.18% to 6.30%. Conclusions. Unsaturated fatty acids are predominant in lipid composition of the studied bacteria. The studied bacteria were identified as E. italicus based on the spectrum of fatty acids.
Authors and Affiliations
A. G. Merlich, N. V. Korotaieva
Keywords
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- EP ID EP396754
- DOI 10.18524/2307-4663.2018.3(43).142237
- Views 100
- Downloads 0
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