Experimental study on the application of injectable concentrated growth factors gel in dental pulp regeneration of permanent teeth
Journal Title: Journal of Air Force Medical University - Year 2023, Vol 44, Issue 9
Abstract
Objective To investigate the effect of injectable concentrated growth factors (CGF) gel on dental pulp regeneration of permanent teeth in beagles and the balance between new tissue mineralization and pulp-like tissue formation in pulp regeneration. Methods The pulp infection model of beagles was established firstly. Then, the pulp tissue was removed and the root canal was prepared. The anterior teeth of beagles were randomly divided into four groups: empty canal group (only removal of the pulp tissue), revascularization group (stimulation of apical bleeding to fill the root canal), CGF group (injection of CGF gel into the root canal to fill the root canal), and revascularization + CGF group (stimulation of apical bleeding to fill the root canal by 1 / 2, injection of CGF gel into the root canal by full length). The root canal was closed with iRoot-BP Plus in each group, and the crown was filled with flow resin. X-radiographs were taken before surgery and 3 months after surgery to observe periapical image changes, and histological observation was made 3 months after surgery. Results X-radiographs showed that no obvious apical shadow was observed before and after pulp regeneration in each group, but blurred hard plate of periapical bone was observed in the empty canal group. HE staining showed that no significant new tissue was observed in the root canals of the empty canal group. In the revascularization group, the new tissue reached about 1/ 2 of the root canal, and was mainly bone-like tissue. In the CGF group, the new tissue in the root canal was mainly loose connective tissue with vascular structure, which could reach about 2 / 3 of the root canal. In the revascularization + CGF group, the new loose connective tissue in the root canal was similar to the healthy pulp tissue, with multicellular layer and cell-free layer, and the vascular structure was evenly distributed. A mineralization bridge was formed in the crown part of the root canal which wrapped the new pulp tissue. In addition to the empty canal group, neonatal dentin-like tissue could be seen in the root canal wall of the other three groups. Conclusion Injectable CGF gel can promote pulp tissue regeneration, and its combination with revascularization can regulate the balance between new tissue mineralization and pulp tissue formation.
Authors and Affiliations
WANG Minghao, GUO Qian, ZHANG Yidan, ZHU Xiaomiao, JIANG Wenkai, WANG Shengchao, HE Wenxi
Keywords
Related Articles
- EP ID EP724203
- DOI -
- Views 21
- Downloads 0
Relationship between inflammatory indexes in peripheral blood of COPD patients at stable stage and comprehensive assessment of disease severity
Objective To observe the expression of cytokines and inflammatory indexes in peripheral blood of patients with chronic obstructive pulmonary disease ( COPD) in different groups at stable stage, and analyze their correla...
Visualization analysis of SGLT2 inhibitors in the treatment of heart failure based on CiteSpace
Objective To visually analyze the number of papers, authors, research institutions and keywords related to sodium-glucose cotransporter 2 (SGLT2) inhibitors in the treatment of heart failure ( HF) by using CiteSpace, an...
Protective effect of pentoxifylline on human umbilical vein endothelial cell injury induced by tert-butyl hydroperoxide
Objective To investigate the protective effect of pentoxifylline (PTX) on oxidative stress and mitochondrial function in human umbilical vein endothelial cells (HUVECs) induced by tert-butyl hydroperoxide (t-BHP). Metho...
Design of a portable full-automatic cleaning and disinfection device for oral impression
Objective To design a portable full-automatic cleaning and disinfection device for oral impression, so as to realize rapid, effective and full-automatic cleaning and disinfection of oral impression. Methods The device w...
Investigation and evaluation of dietary nutrition of cadets in an Air Force college during field training
Objective To understand the dietary intake of cadets in an Air Force college during field training and carry out nutritional analysis, so as to provide a reference for the military dietary nutrition support. Methods Die...