EXPLORATION OF VARIOUS CLASSICAL AND CHEMOMETRIC ASSISTED UV SPECTROPHOTOMETRIC METHODS FOR ESTIMATION OF CHLORHEXIDINE GLUCONATE AND CETRIMIDE IN BULK AND ITS FORMULATION
Journal Title: Indo American Journal of Pharmaceutical Research - Year 2017, Vol 7, Issue
Abstract
Abstract The present work inculcates with the approach for development of various classical and chemometrics assisted UV spectrophotometric methods for estimation of Chlorhexidine gluconate (CH) and Cetrimide (CET) in bulk and its formulation. The methods developed herein include simple Simultaneous equation method (Vieordt’s method), First Derivative spectroscopy method, Multicomponent analysis method, Classical least squares, Inverse least square, Partial least squares, Absorption ratio spectra method and Mean centering of ratio spectra method. The developed methods were successfully validated according to ICH Q2 (R1) guidelines. All methods showed a good linear response. The mean percentage assay values for all UV methods were found to be in the range of 98-102%. Statistical analysis was also applied for assay results of the developed methods which included One-way ANOVA and post hoc analysis like Tukey Honest significant test and Scheffe multiple comparison test. The major outcome of research imbibes to be developed analytical methods were found to be specific, selective, and robust and can be applied for routine analysis of marketed formulation in laboratory premises. Thus, various novel and simple UV analytical methods were explored and available for analysis of the selected drugs.
COMPREHENSIVE REVIEW OF IMPORTANT ANALYTICAL REAGENTS USED IN SPECTROPHOTOMETRY
Abstract An innovative and comprehensive range of chromogenic reagents or colorimetric reagents are generally used for the application in the field of spectroscopic quantitative and qualitative analysis. There are number...
GREEN SYNTHESIS OF ZINC OXIDE NANOPARTICLES USING RUBIA CORDIFOLIA ROOT EXTRACT AGAINST DIFFERENT BACTERIAL PATHOGENS
Abstract Wide application of nanoparticles stimulates the need for synthesizing them But, the conventional methods are usually hazardous and energy consuming. This lead to focus on “green synthesis”of nanoparticles which...
A REVIEW ON SOLID DISPERSIONS DRUG DELIVERY SYSTEMS
Solid dispersions have attracted considerable interest as an efficient means of improving the dissolution rate and hence the bioavailability of a range of hydrophobic drugs. This article reviews the various preparation t...
ANALYTICAL METHOD DEVELOPMENT AND VALIDATION FOR THE ESTIMATION OF VILDAGLIPTIN IN BULK AND ITS DOSAGE FORM USING UV SPECTROPHOTOMETER
A simple, rapid, precise and economical Analytical method development has been developed for quantitative vildagliptin in manufactured tablet formulation. The stock solution and subsequent dilution of vildagliptin was do...
EXTENDED RELEASE TABLET FORMULATION OF A MACROLIDE ANTIBIOTIC
Abstract Clarithromycin is a macrolide antibiotic. The objective is to design the extended release tablet of Clarithromycin which will exhibit comparative in-vitro and in-vivo drug release profile as that of marketed pro...