Expression of hsa Let-7a MicroRNA of Macrophages Infected by Leishmania Major
Journal Title: International Journal of Medical Research & Health Sciences (IJMRHS) - Year 2016, Vol 5, Issue 10
Abstract
Leishmaniasis is a vector-born disease caused by species of the genus Leishmania and is transmitted from host to host through the bite of an infected sandfly. MicroRNAs (miRNAs) are non-coding small RNAs with 22-nucleotide length. They are involved in some biological and cellular processes. We aimed to evaluate the expression of let-7a in human macrophages miRNA when are infected by Leishmania major. We also evaluated the impact of Leishmania major infection on the expression of let-7a at two different times, 24 and 48 hours, after infection. Blood samples were collected from ten healthy volunteers with no history of leishmaniasis. Development of macrophages from peripheral monocytes and infection with stationary phase of Leishmania major promastigotes were done through serial cultures under 5% CO2 environment and 37C.To measure the expression levels of let-7a real-time PCR was performed with specific related primers using the SYBR® Green master mix Kit™. The real-time PCR showed let-7a was expressed in cells infected with parasites after 24 and 48h post-infection. Comparison of let-7a miRNA expression after 24 and 48 h revealed that let-7a miRNAs were down-regulated at 48 h post-infection more than 24h after infection. The results of this study suggest that according to the main function of miRNA in repression of mRNA translation it could be possible to manipulate host cells in order to alter miRNA levels and regulate macrophage functions after establishment of intracellular parasites such as Leishmania.
Authors and Affiliations
Nooshin Hashemi| Department of Parasitology & Mycology, School of Medicine, Isfahan University of Medical Sciences , Isfahan, Iran, Mohammadreza Sharifi| Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran, Sepideh Tolouei| Department of Parasitology & Mycology, School of Medicine, Isfahan University of Medical Sciences , Isfahan, Iran, Mitra Hashemi| Deputy of Research and Technology, North Khorasan University of Medical Sciences, Bojnurd, Iran, Cyrus Hashemi| Dentist,North Khorasan, Social Security Organization, Bojnurd, Iran, Seyed Hossein Hejazi| Skin Diseases and Leishmaniasis Research Center, Department of Parasitology & Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran, Corresponding E-mail: hejazi@med.mui.ac.ir
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