Extraction of Secondary Metabolites from Roots of Acanthus Ilicifolius L and Screening for Antioxidant and Antibacterial Activity.
Journal Title: International Journal of Pharmaceutical Science Invention - Year 2017, Vol 6, Issue 2
Abstract
The root extracts of Acanthus ilicifolius L finds a prominent place in folk medicine. In this study, we extracted alkaloid, flavonoid, tannin and total phenols in benzene, ethyl acetate, acetone, methanol and ethanol, their antibacterial activity and antioxidant activity was evaluated. The antioxidant activity is executed by FRAP assay and agar well diffusion method is done to study the antibacterial activity against Enterobacter aerogenes, Enterobacter cloacae, Escherichia coli, Bacillus subtilis, Staphylococcus aureus and Streptococcus pyogenes. The antibacterial activity of all the extracts was compared with standard antibiotic gentamicin. The Minimum Inhibitory Concentration [MIC] was determined by serial dilution method. Alkaloids are rich in acetone and Flavonoids are high in methanol extracts. The acetone extract showed higher antioxidant activity, while benzene extract was identified to contain lower antioxidant activity. The extent of inhibition by the root extracts diverge between the solvents used, among them ethanol extracts exhibited higher level of inhibition against the gram positive test cultures compared to gram negative test cultures employed. Whereas, the acetone extracts efficacy is more on gram negative test cultures than the gram positive cultures. The MIC was found to be between 1mg/100µl to 5mg/100µl. This study gives the source for purification and characterization of bioactive principles that possess antioxidant and antibacterial action from the root of Acanthus ilicifolius.
Authors and Affiliations
N. Sharief Mohammad, Mathewos Genetoa, Dejene Deresh Abateneh, Mohammad Salahuddin, Mohammad Dilshad Manzar, Uma Maheswara Rao V
Keywords
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