Extracts of Adhatoda vasica Nees. and vasicine inhibits biofilm formation of Klebsiella pneumoniae
Journal Title: IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS) - Year 2018, Vol 13, Issue 2
Abstract
Adhatoda vasica Nees. are used traditionally in Tripura to cure pneumonia, cough and cold. Pneumonia is mainly caused by bacteria like Klebsiella pneumoniae. Moreover, many of these pathogenic strains are showing acute resistance for many commercially available antibiotics due to its biofilm formation ability. The present study evaluates the antimicrobial activity of Adhatoda vasica Nees. extracts against the biofilms of Klebsiella pneumoniae and their free-living forms. After extraction and phytochemical screening of Adhatoda vasica Nees, the presence of vasicine in extracts was ascertained by thin layer chromatography. The antimicrobial activity for all the extracts and commercially available vasicine against planktonic forms of bacteria were determined using the agar well diffusion method with tetracycline as control. The minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) values were evaluated by a macrobroth dilution technique. The anti-biofilm effects were assessed by microtitre plate method. The cell viability assay was assessed by EtBr/AO staining. Results shows that the A. vasica Nees. leaf extract have remarkable zone of inhibition for Klebsiella pneumoniae as compared to standard vasicine and tetracycline. Similarly, the MIC values confirmed its outstanding ability for inhibition of planktonic as well as biofilm form of the bacteria, and same is also been visualized and established further by cell viability studies and SEM analysis for the biofilm of Klebsiella pneumoniae .
Authors and Affiliations
Lovely Rahaman, Sushanta Ghosh, Nandita Nath, Aparna Sen, Kiran Sharma, Bipin Kumar Sharma
Keywords
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- EP ID EP394265
- DOI 10.9790/3008-1302013749
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