Fixation in tissue processing

Journal Title: International Journal of Farming and Allied Sciences - Year 2013, Vol 2, Issue 18

Abstract

The present study is about Fixation of tissue. It is done for several reasons. One reason is to kill the tissue so that postmortem decay (autolysis and putrefaction) is prevented (Carson and Hladik, 2009). Fixation preserves a sample of biological material (tissue or cells) as close to its natural state as possible in the process of preparing tissue for examination. To achieve this, several conditions usually must be met. In the fields of histology, pathology, and cell biology, fixation is a chemical process by which biological tissues are preserved from decay, thereby preventing autolysis or putrefaction. Fixation terminates any on going biochemical reactions, and may also increase the mechanical strength or stability of the treated tissues.First, a fixative usually acts to disable intrinsic biomolecules particularly proteolytic enzymes—which otherwise digests or damages the sample. Second, a fixative typically protects a sample from extrinsic damage. Fixatives are toxic to most common microorganisms (bacteria in particular) that might exist in a tissue sample or which might otherwise colonise the fixed tissue. In addition, many fixatives chemically alter the fixed material to make it less palatable (either indigestible or toxic) to opportunistic microorganisms. Finally, fixatives often alter the cells or tissues on a molecular level to increase their mechanical strength or stability. This increased strength and rigidity can help preserve the morphology (shape and structure) of the sample as it is processed for further analysis.

Authors and Affiliations

Hamed Ganjali, Mahla Ganjali

Keywords

Related Articles

Soil characteristic for sustainable vegetables farming in Noongan Sub-watershed area, Minahasa North Sulawesi

The vegetable farming sustainability is influenced by soil quality. The objective of the research is to determine soils characteristics for vegetable farming in Noongan sub-watershed, Minahasa. Research was done by soi...

The effect of two types of bio-fertilizers on the tuber production of potato: (a case study of Damavand area)

Potato third of agricultural production in the country has after wheat and rice. But because of the damage caused by viral pathogens that performance per unit area decreases. The use of biological fertilizers to increa...

Effective DNA Extraction Protocol for Kenaf

Polymerase chain reaction (PCR) has a wide range of applications in molecular biological research. However, the quantity and quality of template DNA have a significant consequence on the reproducibility of PCR results....

The effect of vermicompost on the forming ingredients of calendula officinal is L herb´s essence

One experiment was carried out for different levels of vermicompost on the forming ingredients of calendula officinal is L herb´s essence in form of totally random design with 4 treats (2%, 4%, and 6%) with 3 replicati...

Removal of Nacl from aqueous solutions by using clinoptilolite

This study focuses on the absorption of sodium chloride via clinoptiolite. The effect of different parameters is investigated like concentration, adsorbent dosage and particle size. The results showed that, by decreasi...

Download PDF file
  • EP ID EP32378
  • DOI -
  • Views 315
  • Downloads 0

How To Cite

Hamed Ganjali, Mahla Ganjali (2013). Fixation in tissue processing. International Journal of Farming and Allied Sciences, 2(18), -. https://europub.co.uk/articles/-A-32378