Fixation in tissue processing
Journal Title: International Journal of Farming and Allied Sciences - Year 2013, Vol 2, Issue 18
Abstract
The present study is about Fixation of tissue. It is done for several reasons. One reason is to kill the tissue so that postmortem decay (autolysis and putrefaction) is prevented (Carson and Hladik, 2009). Fixation preserves a sample of biological material (tissue or cells) as close to its natural state as possible in the process of preparing tissue for examination. To achieve this, several conditions usually must be met. In the fields of histology, pathology, and cell biology, fixation is a chemical process by which biological tissues are preserved from decay, thereby preventing autolysis or putrefaction. Fixation terminates any on going biochemical reactions, and may also increase the mechanical strength or stability of the treated tissues.First, a fixative usually acts to disable intrinsic biomolecules particularly proteolytic enzymes—which otherwise digests or damages the sample. Second, a fixative typically protects a sample from extrinsic damage. Fixatives are toxic to most common microorganisms (bacteria in particular) that might exist in a tissue sample or which might otherwise colonise the fixed tissue. In addition, many fixatives chemically alter the fixed material to make it less palatable (either indigestible or toxic) to opportunistic microorganisms. Finally, fixatives often alter the cells or tissues on a molecular level to increase their mechanical strength or stability. This increased strength and rigidity can help preserve the morphology (shape and structure) of the sample as it is processed for further analysis.
Authors and Affiliations
Hamed Ganjali, Mahla Ganjali
Keywords
Related Articles
- EP ID EP32378
- DOI -
- Views 289
- Downloads 0
The role of plants in refinement of heavy metals in soil
Due to the increasing industrial activity in recent years and the subsequent entry of inorganic and organic contaminants in soil, including the problems that threaten human health. Phytoremediation is used by Green Pla...
The Effect of silver nanoparticle on lentil Seed Germination under drought stress
A study was conducted to evaluate the effect s of silver nanoparticles for ameliorating negative effects of drought stress on germination and growth of lentil seeds. In order to investigate drought stress on lentil ger...
Yield changes and wheat remarkable traits influenced by salinity stress in recombinant inbred lines
More than six percent of areas in the world, or about fifty percent of the land area of wet land face with salinity problems. In Iran, saline soils area is over 18.5 million hectare. Since water resources with good qua...
Investigating Thickness and Porosity of Covering Layer of Chitosan on Pistachio Kernel
The main aim of this study was finding a new method to prevent aflatoxin hazard in pistachio kernels. In this study qualitative and quantitative properties of pistachio kernels covered with concentrations 260 ppm, 500...
Effect of weeding and by Forage and Medicinal plants as Companion Crops on Some of quality traits of Corn (Zea mays)
In order to study the effect of weeding and by forage and medicinal plants as companion crops on some of quality traits of corn, an experiment was conducted with corn and cover crops at Agricultural Research Station of...