Fixation in tissue processing
Journal Title: International Journal of Farming and Allied Sciences - Year 2013, Vol 2, Issue 18
Abstract
The present study is about Fixation of tissue. It is done for several reasons. One reason is to kill the tissue so that postmortem decay (autolysis and putrefaction) is prevented (Carson and Hladik, 2009). Fixation preserves a sample of biological material (tissue or cells) as close to its natural state as possible in the process of preparing tissue for examination. To achieve this, several conditions usually must be met. In the fields of histology, pathology, and cell biology, fixation is a chemical process by which biological tissues are preserved from decay, thereby preventing autolysis or putrefaction. Fixation terminates any on going biochemical reactions, and may also increase the mechanical strength or stability of the treated tissues.First, a fixative usually acts to disable intrinsic biomolecules particularly proteolytic enzymes—which otherwise digests or damages the sample. Second, a fixative typically protects a sample from extrinsic damage. Fixatives are toxic to most common microorganisms (bacteria in particular) that might exist in a tissue sample or which might otherwise colonise the fixed tissue. In addition, many fixatives chemically alter the fixed material to make it less palatable (either indigestible or toxic) to opportunistic microorganisms. Finally, fixatives often alter the cells or tissues on a molecular level to increase their mechanical strength or stability. This increased strength and rigidity can help preserve the morphology (shape and structure) of the sample as it is processed for further analysis.
Authors and Affiliations
Hamed Ganjali, Mahla Ganjali
Keywords
Related Articles
- EP ID EP32378
- DOI -
- Views 318
- Downloads 0
Study on the process of herbicide degradation in paddy soils of Mazandaran province
Herbicides are important for Grass weeds control but herbicide usage is one of the most important subjects in contaminants soil and water of fields. The length of time that the herbicides in soil is stable and active f...
Software Programs for analyzing genetic diversity
Initial selection and individual selection is basic in genetic diversity studies of vast populations. The selection can be done by some molecular techniques like molecular marker .This will be resulted in saving time a...
Evaluation of Betanal Progress of herbicide effects on root morphological traits of sugar beet
This study was conducted to evaluation of Betanal Progress OF herbicide on root morphological traits of sugar beet. The experimental design was factorial on the basis of randomized complete block with four replications...
Grain filling pattern by Measurement of Indolyl-3-acetic acid, Gibberellin and cytokinin content in the grains
Grain filling patterns and their relationships with indole-3-acetic acid (IAA) and gibberellin (GA contents in the grains and roots during grain development were examined in Grain growth rate (GGR), gibberellins, cytok...
Socio-Economic Role of Agricultural Cooperatives in S&B Province
Agriculture is mother of all industries and it plays an important role in socio-economic life of developing and agricultural oriented countries. Developing countries like Iran with agricultural base but exporting oil a...