Flow cytometry in diagnostics of neutropenia
Journal Title: Postępy Nauk Medycznych - Year 2012, Vol 25, Issue 7
Abstract
Polymorphonuclear granulocytes, neutrophils, are the most important cellular components of innate immune response. They are produced in bone marrow during a complex differentiation process from multipotential stem cells. Individual differentiation stages differ in morphology, proliferating ability and metabolic features. Inadequate number or activity of neutrophils is manifested by infections, which may be extremely severe.<br>Neutropenia composes a heterogeneous group of disorders with increased susceptibility to bacterial infections. Most usually, neutropenia is drug-related or secondary to environmental factors or pathogens. It may be also congenital, associated with defective granulopoiesis due to maturation defects, increased apoptosis or myelokathexis. Differences in expression of surface antigens among individual myeloid maturation stages make flow cytometry a useful tool in evaluation of granulopoiesis and its defects, as well as diagnostics of neutropenia associated with other cellular disorders. The technique allows also evaluation of efficiency of killing mechanisms, as well as detection of anti-granulocyte antibodies causing immune neutropenia. Selected examples of application of flow cytometry in diagnostics of congenital and immune neutropenia have been presented.<br>Complexity of pathomechanisms leading to chronic neutropenia makes flow cytometry a very useful tool, providing pieces of information complementary to classical morphologic studies. It allows analysis of very large number of myeloid cells, simultaneous examination of other cell lines, as well as evaluation of activity of enzymes, leading to significant reduction of time necessary to obtain correct diagnosis and implementation of adequate therapy.
Authors and Affiliations
Barbara Piątosa, Maja Klaudel-Dreszler
Keywords
Related Articles
- EP ID EP54494
- DOI -
- Views 119
- Downloads 0
Constipation in the elderly – diagnostic and therapeutic differences
Constipation perceivable as a stool frequency lower than typical for a given population and/or disordered strained defecation occurs in any age, but its prevalence in older persons (> 65 years) is five times more...
Adolescent scoliosis with atypical rapid progression – a case report study
Adolescent idiopathic scoliosis in immature patient is likely to progress when exceeds 30 degrees of Cobb, however the progression rate is usually moderate, amounting to several degrees per year.<br>The aim...
Alloprzeszczepienie komórek krwiotwórczych w opornych przypadkach chłoniaka Hodgkina szansą na długotrwałą remisję
Skuteczność alloprzeszczepienia komórek hematopoetycznych (alloHSCT) w leczeniu chłoniaka Hodgkina (HLy) jest nadal dyskutowana.<br>W Klinice Hematologii i Transplantacji Szpiku Śląskiego Uniwersytetu Medyc...
Abdominal trauma before the onset of chronic pancreatitis – cause of disease or fortuity?
Introduction. The most common causes of chronic pancreatitis (CP) in children differ significantly from those reported in adults. The most important are gene mutations, anatomic anomalies of pancreatic ducts, lipid disor...
Prof. dr hab. Zygmunt Wincenty Górka – życiorys