Abstract

The nanoparticles containing Antihypertensive agent (Amlodipine besylate) and Hypolipidaemic drug (Atorvastatin calcium) were prepared by nanoprecipitation technique using PLGA, Eduragit RLPO as polymers and pluronic F 68 as tribloere polymeric stabilizer. The preformulation studies were carried out to confirm the compatibility of with excipients by FT – IR and for drug, solubility studies, Hygroscopicity, loss on drying for drug identification. The prepared nanoparticles were assayed by HPLC to determine the drug content. The morphological shape was confirmed by using Scanning Electron Microscope. The particle size distribution was analyzed by using particle size analyzer. The average mean particle size of F1, F2, F3, F4 were 50nm, 70nm, 80nm and 100nm respectively. For Atorvastatin: The entrapment efficiency of formulation F2 containing PLGA 5 mg and pluronic F68 was found to be 69% which showed maximum percent drug entrapment where as those containing (F1) PLGA 10mg, (F3) Eudragit 10 mg and (F4) Eudragit 5 mg were found to be 65, 45,47 respectively. For Amlodipine: The entrapment efficiency of formulation F2 containing PLGA 5 mg and pluronic F68 was found to be 74% which showed maximum percent drug entrapment where as those containing PLGA 10 mg (F1), Eudragit 10 mg (F3) and Eudragit 5 mg (F4) were found to be 72, 51, 54 respectively. Therefore, the formulation F2 showed maximum drug entrapment efficiency for both drugs Atorvastatin and Amlodipine. For Atorvastatin: The % amount released for F1, F2, F3, F4 at 48 hours were found to be 90.15%, 97.44% 74.07%, 76.32% respectively. The maximum % amount released was observed for F2 when compared to all other formulation. For Amlodipine: The % amount released for F1, F2, F3, F4 at 48 hours were found to be 92.58% 95.33%, 84.19% 84.19% respectively. The maximum percentage amount release was more for F2 when compared to other three formulations. So formulation F2 produced more drug release when compared to F1, F3 and F4. From the release studies, the polymer Eudragit was more sustaining action when compared to PLGA. According to my work, the formulation containing antihypertensive agent should not be sustaining for long time. So I conclude that the PLGA was best polymer for maximum % drug release at 48 hours than the Eudragit RLPO.The formulation F1 showed zero order release3 kinetics, and F3, F4 did not fitted for any other release kinetics. But, release kinetics of F2 indicated that it follow zero order and Higuchi model rather than first order. These findings indicated that

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