Genetic Diversity Analysis of Date Palm (Phoenix dactylifera L.) Genotypes using RAPD Markers
Journal Title: Annual Research & Review in Biology - Year 2015, Vol 5, Issue 1
Abstract
Date palm (Phoenix dactylifera L.) is one of the most important fruit crops in the Middle East. In the present research, RAPD DNA marker was used to characterize six date palm genotypes grown in the Jiroft region of Iran. The pre-screening of 18 primers on genotypes allowed selection of 16 primers which revealed polymorphism and gave reproducible results. All analyzed genotypes were distinguishable by their band patterns. Cluster analysis by UPGMA showed two main clusters for palm genotypes based on difference in fruit color with variation in sexuality and early or mid-season maturities. Mozafati, Kabkab and Mordarsang genotypes were located on the first cluster, while the second cluster consisted of Kaloteh, Male211 and Male504 genotypes, which were most closely related with the highest value in conformity with Nei and Li's similarity value of 0.58. Dendrogram was confirmed as valuable cluster Principal Coordinate Analysis (PCoA) using the first principal component from the molecular data. The results of molecular analysis were adapted with genetic variation. RAPD technology therefore appears very effective for identifying genotypes, polymorphism and genetic distance of date palm.
Authors and Affiliations
Alireza Bahraminejad, Ghasem Mohammadi-Nejad
Keywords
Related Articles
- EP ID EP353403
- DOI 10.9734/ARRB/2015/11683
- Views 119
- Downloads 0
Diverse Genetic Screening and Counseling throughout the Iranian Population
Background and Aim: The recent decades have witnessed increasing possibilities for genetic testing and screening. In the Iran, since the 1970s, individuals and their family members could obtain genetic counselling for th...
Bioconversion of Sweet Potato Leaves to Animal Feed
Background: The high cost of conventional animal feed ingredients in Nigeria has made it necessary to search for alternative local sources of feed. Crop residues including sweet potato leaves abound in Nigeria. These hav...
Effect of Metarhizium anisopliae on Different Group Size of Coptotermes heimi
Coptotermes heimi is a destructive subterranean termite species commonly found on the Indian subcontinent. Environmental concerns of chemical insecticides usage forced scientist to concentrate on alternative methods. Met...
Evaluation of Passiflora edulis Leaf Sample Storage Methods on RNA Quality and Suitability for Use in RT-PCR Assays
It is prerequisite to extract Ribonucleic Acid (RNA) with high quality and integrity in order to carry out molecular biology studies in any plant species. Samples collected from remote fields require preservation before...
Association of 10 p Locus with Slovenian Inflammatory Bowel Disease Patients
Aim: To evaluate association of 10 p locus in Slovenian inflammatory bowel disease (IBD) patients with different sub-phenotypes. Methodology: Genotyping of three selected SNPs from 10 p locus was performed in 594 IBD pat...