Genotype Distribution of Human Papillomavirus in Women with Cervical Cancer and Normal Cytology in Malwa Region of Punjab- A Pilot Study
Journal Title: Journal of Clinical and Diagnostic Research - Year 2018, Vol 12, Issue 12
Abstract
ABSTRACT Introduction: Human Papillomavirus (HPV), a small circular double stranded DNA virus is the major aetiological agent of cervical precancerous and cancerous lesions. Genital HPV types are divided into high and low risk types, according to their oncogenic potential. The proportion of women infected with HPV varies greatly across populations as is the distribution of HPV genotypes. Aim: The present pilot study was conducted to determine the most frequent genotypes of HPV associated with: a) Histologically proven cases of cervical carcinoma; b) Women with normal cervical cytology. Materials and Methods: The present hospital based crosssectional study was conducted on 132 women attending Obstetrics and Gynaecology department of a tertiary care hospital. Consecutive cervical brushings were collected from women presenting with any type of cervical lesion and were subjected to cytology, histopathological examination, HPV DNA testing and genotyping by using Sacace real time PCR. Results: Of the 132 women studied between January 2014- October 2014, 50 (37.87%) had histologically proven cervical carcinoma and 82 (62.12%) had normal cytology. In proven cases of cervical carcinoma, HPV infection was found to be associated in 49/50 (98%) and all of them had high risk HPV genotypes. The most common genotypes were 16 (67.34%) and 18 (20.4%). Other genotypes present were 45 and 33. Out of 82 cytological normal women, five showed presence of HPV DNA on RT PCR. While all the five had infection of HPV genotype 18, in one there was presence of both 16 and 18 genotypes. Conclusion: The detection of HPV genotypes 16, 18 along with high risk type 33 and 45 in Malwa region of Punjab confirm the importance of identifying the types of HPV that infects this community to design more effective prevention strategies and thus contribute to fight against cervical cancer.
Authors and Affiliations
Shilpa Arora, Avnisha . , Pragati Grover, Navneet kaur, Neerja Jindal, Ratika Gupta
Keywords
Related Articles
- EP ID EP525833
- DOI 10.7860/JCDR/2018/37070.12361
- Views 38
- Downloads 0
Efficacy of Task Oriented Exercise Program Based on Ergonomics on Cobb’s Angle and Pulmonary Function Improvement in Adolescent Idiopathic Scoliosis- A Randomized Control Trial
Introduction: Scoliosis is lateral curvature of the spine greater than 100 accompanied by vertebral rotation. The prime risk factors for curve progression are a large curve magnitude, skeletal immaturity and female gend...
Non-osseous Tubercular Lesions of Spinal and Paraspinal Region-Evaluation by MRI
ABSTRACT Introduction: Spinal tuberculosis is very common in developing countries like India and it has a wide spectrum of appearance on MRI ranging from simple marrow oedema to complete vertebral body collapse. Early di...
Effect of Cell Phone Radiations on Orofacial Structures: A Systematic Review
Introduction: The widespread use of cell phone in recent years has raised many questions whether their use is safe to operator who is exposed to Electromagnetic Waves (EMV). Aim: To find out the effect of cell phone emit...
Pitfalls in Diagnosing Seropositive Rheumatoid Arthritis: Haematologic Malignancy Presenting as an Autoimmune Disease
ABSTRACT Carcinomatous Polyarthritis (CP) is defined as the development of arthritis in association with a malignancy but distinct from that associated with metastasis or direct invasion. It can occur before, with, or af...
Metabolic Syndrome and Cardiovascular Disease Risk in patients with Depressive disorder on Antidepressive Medication
ABSTRACT Introduction: Depression and Metabolic syndrome both are the two major public health issues. The depressive patients are at higher risk for Cardiovascular Disease (CVD). The use of antidepressant medication has...