Genotyping and prevalence of hepatitis c virus in dialysis patients in Gandhi general hospital - A tertiary care centre
Journal Title: IP International Journal of Medical Microbiology and Tropical Diseases - Year 2018, Vol 4, Issue 2
Abstract
Context The present study was undertaken to determine the genotyping and prevalence of hepatitis c virus in dialysis patients as HCV genotyping has become a part of the pretreatment evaluation Studies correlating risk factors and genotypes of HCV infection prevalent in Haemodialysis patients are scanty Therefore our study is conducted to study the prevalence genotypes and risk factors in order to detect and try to curb HCV infectionAims To study the genotypes of hepatitis C infection and its prevalence in hemodialysis patientsSettings and Design and Period Study was conducted in Gandhi hospital Secunderabad in 1 year period as Crosssectional studyMaterials and Methods A total of 225 serum samples were collected from chronic renal failure patients who were undergoing Haemodialysis over a period of 1 year The samples were screened for anti HCV antibodies by third generation ELISA test 25 antiHCV positive samples and 40 antiHCV negative samples were randomly selected for HCV RNA detection using TrizolChloroformIsopropyl alcohol method HCV RNA was reverse transcribed with 200 U of maloney murine leukemia virus reverse transcriptase and random hexamers Nested PCR was done HCV RNA positive samples were genotyped with primers specific for core region of different HCV types by type specific PCRStatistical Analysis Used Statistical analysis of the data was done by chisquare c2 test using EPIINFO 2000 software The values of chi square test are interpreted as not significant gt005 highly significant lt 0001Results Out of 225 hemodialysis patients 38 168 patients were anti HCV positive Duration of dialysis was significantly longer in antiHCV antibodies positive group with dialysis duration more than 2 years Seropositivity is more in HD patients having dialysis at more than one centre HCV RNA was detected in randomly selected 1325 52 anti HCV positive patients and in 540 125 anti HCV negative patients The genotype distribution was as 3a 611 2a 1111 2b 55 mixed genotypes 166 untypable 55Conclusions Duration of dialysis number of centres used for dialysis and number of times dialysis was done are important associations for antiHCV antibodies positivity Genotype 3 was predominant 6111 Detection of genotypes helps in early initiation of specific therapy and helps in early prediction of prognosis in patients of chronic renal failure on hemodialysisKeywords Chronic renal failure Seropositivity
Authors and Affiliations
Kasawar Darahasa, Syeda Amtul Moqueeth
Keywords
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- EP ID EP485848
- DOI 10.18231/2581-4761.2018.0018
- Views 59
- Downloads 0
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