Ginkgetin or Isoginkgetin: The Dimethylamentoflavone of Dioon edule Lindl. Leaves

Journal Title: European Journal of Medicinal Plants - Year 2016, Vol 16, Issue 3

Abstract

Aims: Phytochemical study of the biflavonoid content of Dioon edule Lindl. leaves in addition to quantification of the phenolic content and evaluation of their antioxidant potential. Methodology: Chromatographic isolation of the total alcohol extract of the leaves followed by the spectroscopic identification of the isolated compounds was performed adopting 1D, 2D NMR techniques. The total phenolic content (TPC) was determined using the Folin-Ciocalteu method and the total flavonoids content (TFC) was measured by complexation with aluminum chloride and the antioxidant activity was evaluated with DPPH (2.2-diphenyl-1-picrylhydrazyl) assay. Results: Phytochemical investigation of Dioon edule Lindl. leaves afforded the isolation of 7,4′,7″,4″′-tetramethylamentoflavone (1), 4′,4″′-dimethylamentoflavone (isoginkgetin) (2) biolbetin (3) and amentoflavone (4) in addition to β-sitosterol (5). A more accurate assignment of 13C-NMR data of the methylated amentoflavone derivatives is performed using 2D NMR (HMQC and HMBC). Compounds 2 and 5 are reported for the first time in Dioon edule Lindl. leaves. Previous report on D. edule Lindl. leaves afforded the isolation of ginkgetin (6) as a dimethylamentoflavone but our investigation proved that it is isoginkgetin. The TPC was 0.27±0.01 gallic acid equivalents in mg/g plant material. The TFC was 1.8473±0.077 rutin equivalents in mg/g plant material. The plant exhibited a good antioxidant property as DPPH scavenging activity was 3.29±0.251 mg ascorbic acid equivalent antioxidant capacity in 1 g plant material. Conclusion: The phytochemical study of D. edule Lindl. leaves afforded isoginkgetin instead of the previously reported ginkgetin. The use of NMR provides the most powerful tool for a more accurate assignment of biflavonoids.

Authors and Affiliations

Abeer Moawad, Dalia El Amir

Keywords

Related Articles

Cytotoxic Effect and Chemical Composition of Inula viscosa from Three Different Regions of Morocco

Aims: Worldwide, growing interest was given to find new cytotoxic agents that could be used in breast cancer treatment. In this context, we have planned to evaluate cytotoxic activity of Inula viscosa extracts, on two br...

Anticoccidian Activity of Ethanol Roots Extract of Cassia sieberiana DC in Chickens

Objectives: The aim of this study was to investigate the anticoccidian activity of ethanol roots extract and fractions in chicken experimentally infested by oral administration of coccidia’s oocysts solution. Methods: E...

The Effect of Herbal Medicine against Enterococcus faecalis on Infected Root Canal Substrate: An Ex-vivo Study

Aim: The aim of this study was to evaluate the antimicrobial effects of ginger, cinnamon and their combination against Enterococcus faecalis located in infected root canals. Methodology: A quasi experimental pre-test pos...

Hepatoprotective Activity of Holostemma ada Kodien shcult, Extract against Paracetamol Induced Hepatic Damage in Rats

Aim: To study the hepatoprotective activity of alcoholic extract of Holostemma ada Kodien Shcult against paracetamol (PCM) induced liver damage in rats. Place and Duration of Study: Department of pharmaceutical chemistry...

In vitro Antioxidant Activity and Polyphenolic Content of a Polyherbal Tea and Its Constituents

Aims: Type 2 diabetes mellitus (T2DM) is associated with debilitating co-morbidities, mainly due to chronic hyperglycaemia-induced damage which is mediated by excess reactive oxygen species (ROS). Plant preparations have...

Download PDF file
  • EP ID EP349644
  • DOI 10.9734/EJMP/2016/28560
  • Views 110
  • Downloads 0

How To Cite

Abeer Moawad, Dalia El Amir (2016). Ginkgetin or Isoginkgetin: The Dimethylamentoflavone of Dioon edule Lindl. Leaves. European Journal of Medicinal Plants, 16(3), 1-7. https://europub.co.uk/articles/-A-349644