Hepatoprotective Potential of Methanolic Extract of Gymnemasylvestre Leaves on Acetaminophen-Induced Liver Damage in Wistar Strain Albino Rats
Journal Title: Saudi Journal of Biomedical Research - Year 2018, Vol 3, Issue 1
Abstract
Abstract:Drug-induced liver injury is a potential complication of all medications because the liver has a central role in the metabolism of drugs and toxic substances. This study was designed to evaluate the hepatoprotective activity of the methanolic extract of Gymnemaslyvestreleaves on acetaminophen-induced liver damage in wistar rats. The experiment was carried in three phases. In Phase I, the hepatoprotective activities of the methanolic extract of the leaves was determined by assaying for some liver function indices (AST, ALT & ALP) on acetaminophen-induced liver damage in rats. In phase II, the methanolic extract of the leaves was further fractionated with chloroform, ethylacetate and n-butanol to obtained chloroform, ethylacetate, n-butanol and residual extracts respectively; and the effect of these fractions on liver function (AST, ALT & ALP) was determined. Histopathological examinations were also carried out at each phase of the experiment. From the results, treatment with the methanolic extracts (200mg/kg & 400mg/kg)and the fractions (200mg/kg each) significantly (P<0.05) decreased serum levels of ALT and ALP compared with liver damaged wistar rats, while there was inconsistencies in AST levels after treatment. However, out of all the fractions, the residual fraction showed better hepatoprotective activity. Histopathological examination of the liver showed better architecture and well defined nuclei in rats treated with the methanolic extracts and it fractions while liver of untreated acetaminophen-induced rats was evident of cytolysis. GC-MS analysis of the residual fraction showed the presence of flavonoids, phenolics amongst others. In conclusion, this study has demonstrated the hepatoprotective activity of Gymnemaslyvestre. Keywords:Hepatoprotection; Acetaminophen; Liver;Histopathology; GC-MS; Gymnemasylvestre.
Authors and Affiliations
Oshobu ML, Alhassan AJ, Mansura A, Ononamadu CJ, Ibrahim A
Keywords
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