Highly selective, sensitive and precise Bioanalytical method for the estimation of amoxicillin and clavulanic acid in nano gram range in Albino rat's plasma
Journal Title: Indian Journal of Research in Pharmacy and Biotechnology - Year 2017, Vol 5, Issue 5
Abstract
LC-MS/MS method for the determination of amoxicillin and clavulanic acid in Albino rat's plasma was developed and validated according to currently accepted FDA guidelines of Bioanalytical method validation. The retention times of amoxicillin, clavulanic acid and the internal standard were approximately 0.77, 0.86 and 1.17 minutes, respectively. The overall run time is 3 minutes. The correlation coefficient (r2) is greater than or equal to 0.99. For the within-run accuracy and precision the coefficients of variation for lower limit of quantitation (LLOQ) low (LQC), medium (MQC) and high (HQC) quality control samples were 2.94, 1.31, 5.64 and 6.49 % for amoxicillin and 7.88, 6.79, 5.50, 7.33 for clavulanic acid respectively. The within-run percentages of nominal concentrations for LLOQ, LQC, MQC, and HQC were 94.77, 98.38, 103.18, 104.59 % for amoxicillin and 98.50, 99.83, 101.39 and 97.98 for clavulanic acid respectively.
Authors and Affiliations
K. Vara Lakshmi, A. Sirisha, M. Vinod Kumar, P. Parthiban
Keywords
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