Highly selective, sensitive and precise Bioanalytical method for the estimation of amoxicillin and clavulanic acid in nano gram range in Albino rat's plasma
Journal Title: Indian Journal of Research in Pharmacy and Biotechnology - Year 2017, Vol 5, Issue 5
Abstract
LC-MS/MS method for the determination of amoxicillin and clavulanic acid in Albino rat's plasma was developed and validated according to currently accepted FDA guidelines of Bioanalytical method validation. The retention times of amoxicillin, clavulanic acid and the internal standard were approximately 0.77, 0.86 and 1.17 minutes, respectively. The overall run time is 3 minutes. The correlation coefficient (r2) is greater than or equal to 0.99. For the within-run accuracy and precision the coefficients of variation for lower limit of quantitation (LLOQ) low (LQC), medium (MQC) and high (HQC) quality control samples were 2.94, 1.31, 5.64 and 6.49 % for amoxicillin and 7.88, 6.79, 5.50, 7.33 for clavulanic acid respectively. The within-run percentages of nominal concentrations for LLOQ, LQC, MQC, and HQC were 94.77, 98.38, 103.18, 104.59 % for amoxicillin and 98.50, 99.83, 101.39 and 97.98 for clavulanic acid respectively.
Authors and Affiliations
K. Vara Lakshmi, A. Sirisha, M. Vinod Kumar, P. Parthiban
Keywords
Related Articles
- EP ID EP33491
- DOI -
- Views 289
- Downloads 0
CONTROVERSIAL ROLE OF ANTIPSYCHOTICS IN THE TREATMENT OF ALZHEIMER’S DISEASE
Antipsychotics are the commonly prescribed drugs in the treatment of Alzheimer’s disease, which is the most common form of dementia. Atypical antipsychotics are an effective short-term (6-12 weeks) treatment in relievi...
Invitro anti-arthritic, anti-inflammatory and anti-oxidant activity of Cissus quadrangularis linn
The present investigation deals with the study of in vitro anti-arthritic activity by inhibition of protein denaturation method, anti- inflammatory by HRBC membrane stabilization method and anti-oxidant activity by DPP...
In Silico evaluation of Garciaella species in meor technology
The goal of research using in silico approaches is to study the viability of using microorganisms in the enhanced recovery of oil. A partial 16S ribosomal RNA gene of Garciaella sp. ‘TERI MEOR 02’ with accession number...
STABILITY INDICATING HPLC METHOD FOR THE ESTIMATION OF CINACALCET HYDROCHLORIDE API
A stability-indicating liquid chromatography method has been developed and validated for the determination of Cinacalcet hydrochloride in a laboratory mixture as well as in a tablet formulation developed in-house. Effi...
SYNTHESIS OF NEW THIAZOLIDINE-2,4-DIONE DERIVATIVES AND THEIR ANTIMICROBIAL AND ANTITUBERCULAR ACTIVITY
New 1,3-thiazolidine-2,4-dione (TZD) derivatives 16-29 have been prepared by Knoevenagel condensation reaction between TZD and aromatic aldehydes followed by condensation with 3,4- dichloro benzoyl chloride. The struct...