HPLC ANALYSIS OF BIOACTIVE COMPONENTS OF BLACK CUMIN (NIGELLA SATIVA) AND THEIR RELEVANCE IN FE2+ CHELATION AND OH RADICAL SCAVENGING ABILITY
Journal Title: Mintage journal of pharmaceutical and medical sciences - Year 2017, Vol 6, Issue 4
Abstract
Objective: Black cumin is traditionally used in folklore medicine for the treatment and management of several diseases. The seeds of this plant have been studied for its phytochemical and pharmacological activities. This in vitro research is investigating indigenous total phenol content, the Fe2+ chelation, the hydroxyl (OH) radical scavenging ability of the black cumin seeds aqueous extract spectrophotometrically and to also assess the antioxidant constituents by determining the bioactive compounds. Methods: The bioactive contents were determined using HPLC-DAD analysis while the antioxidant activities were examined using Spectrophotometer. The black cumin seeds were milled into a powder. The powder (5g) was soaked in 100ml of distilled water (1:20 w/v). Results: The study showed that the aqueous extract of black cumin seeds exhibited high antioxidant abilities. Further HPLC-DAD analysis of the cumin seeds revealed a high content of Quercetin>Caffeic acid>Gallic acid>Rosmarinic acid>Chlorogenic acid>Kaempferol>Rutin within the range of (109.42mg/g-5.28mg/g). Conclusion: It could be suggested that the potentials of bioactive components could be further harnessed in the nearest future and adopted for use in the management of degenerative diseases.
Authors and Affiliations
S. A. Shodehinde
Keywords
Related Articles
- EP ID EP436060
- DOI -
- Views 132
- Downloads 0
MOLECULAR INTERACTION OF NATURAL BIFLAVONOIDS, AS COMPETITIVE ANTAGONISTS, WITH AROMATASE RELATED TO BREAST CANCER: AN IN SILICO APPROACH
Objective: Understanding the mechanism of inhibition exerted on aromatase by natural compounds is essential since such compounds may be helpful as a source of efficacious therapeutic agents for the management of postmeno...
ANTIOXIDANT POTENTIAL OF GUNDRUK - AN ETHNIC FOOD OF SIKKIM
Aim: Gundruk is one of the ethnic foods of Sikkim. Ethnic group in Sikkim believe that gundruk is a good appetizer and keeps their body fit and promotes health. In view of this belief it was thought worthwhile to evaluat...
ANTICONVULSANT ACTIVITY OF DIVALPROEX SODIUM AND EFFECT OF GLIMEPIRIDE ON PHARMACOKINETIC: AN EXPERIMENTAL DRUG INTERACTION STUDY
Background and Objectives: Epilepsy is a serious and common chronic neurological disorder caused by abnormal synchronized neuronal discharges. Divalproex is suggested to increase GABA concentration in brain. Both Divalpr...
HYPOLIPIDEMIC EFFECT OF MORINGA OLEIFERA SEED OIL ON HIGH FAT-DIET INDUCED HYPERLIPIDEMIA IN LIVER AND HEART OF ALBINO RATS
Objective: This study investigated the effect of Moringa oleifera seed oil on High fat-diet induced hyperlipidemia in liver and heart of albino rats for a period of 10 days. Methods: Twenty-four albino male Wistar rats...
COMPARING EFFICIENCY OF AHWAZ HOSPITALS BEFORE AND AFTER IMPLEMENTATION OF HEALTH REFORM PLAN BASED ON PABON LASSO MODEL IN 2016
Introduction: Paying attention to health and trying to keep and promote health have been always preferred in health level thus requirements of public and society are met. Pabon Lasso chart is a method to determine effici...