HPLC ANALYSIS OF BIOACTIVE COMPONENTS OF BLACK CUMIN (NIGELLA SATIVA) AND THEIR RELEVANCE IN FE2+ CHELATION AND OH RADICAL SCAVENGING ABILITY
Journal Title: Mintage journal of pharmaceutical and medical sciences - Year 2017, Vol 6, Issue 4
Abstract
Objective: Black cumin is traditionally used in folklore medicine for the treatment and management of several diseases. The seeds of this plant have been studied for its phytochemical and pharmacological activities. This in vitro research is investigating indigenous total phenol content, the Fe2+ chelation, the hydroxyl (OH) radical scavenging ability of the black cumin seeds aqueous extract spectrophotometrically and to also assess the antioxidant constituents by determining the bioactive compounds. Methods: The bioactive contents were determined using HPLC-DAD analysis while the antioxidant activities were examined using Spectrophotometer. The black cumin seeds were milled into a powder. The powder (5g) was soaked in 100ml of distilled water (1:20 w/v). Results: The study showed that the aqueous extract of black cumin seeds exhibited high antioxidant abilities. Further HPLC-DAD analysis of the cumin seeds revealed a high content of Quercetin>Caffeic acid>Gallic acid>Rosmarinic acid>Chlorogenic acid>Kaempferol>Rutin within the range of (109.42mg/g-5.28mg/g). Conclusion: It could be suggested that the potentials of bioactive components could be further harnessed in the nearest future and adopted for use in the management of degenerative diseases.
Authors and Affiliations
S. A. Shodehinde
Keywords
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