HPLC METHOD DEVELOPMENT AND VALIDATION: AN OVERVIEW
Journal Title: Int J of Pharm Res & Analy - Year 2014, Vol 4, Issue 4
Abstract
High performance liquid chromatography (HPLC) is an analytical technique which is proficient to separate, detect and quantify various drugs and its related degradants. An appropriate mobile phase, column, column temperature, wavelength and gradient must be found that affords suitable compatibility and stability of drug as well as degradants and impurities. This review covers the importance of RP-HPLC in analytical method development and their strategies along with brief knowledge of critical chromatographic parameters need to be optimized for an efficient method development.
Authors and Affiliations
G. Santhosh , G. Nagasowjanya , A. Ajitha , Y. Uma Maheswara Rao
ELECTROCHEMICAL REDUCTION BEHAVIOUR AND DETERMINATION OF CEFQUINOME
The electrochemical reduction behaviour of cefquinome has been studied by using dc polarography, cyclicvoltammetry, ac polarography, differential pulse polarography, millicolommetry and controlled potential electro...
SYNTHESIS AND ANTIMICROBIAL ACTIVITY OF 5-{4'-[(4"-ARYL)- -3"-CYANO 2"-METHOXY PYRIDINE-6"-YL] PHENYL CARBAMIDO}-DIBENZ [b,f] AZEPINES
The titled compounds (4a-4k) have been synthesized by the condensation of 5-{4'-[(3"-aryl)-2"-Propene-1"-one]- Phenyl carbamido}-dibenz [b,f] azepines with malononitrile and Sodium methoxide. The biological activities o...
DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR THE ESTIMATION OF TIANEPTINE IN API AND TABLET DOSAGE FORM
ABSTRACT A rapid and precise Reverse Phase High Performance Liquid Chromatographic method has been developed for the validated of Tianeptine, in its pure form as well as in tablet dosage form. Chromatography was...
ANALYTICAL METHOD DEVELOPMENT AND VALIDATION OF SIMULTANEOUS ESTIMATION OF ISOSORBIDE DINITRATE AND HYDRALAZINE HCL IN TABLET DOSAGE FORM BY RP-HPLC
A simple, precise, rapid, specific and accurate reverse phase high performance liquid chromatography method was developed for simultaneous estimation of Isosorbide Dinitrite and Hydralazine HCl in pharmaceutical dosage f...