HUMAN MESENCHYMAL STEM CELLS: A COMPARITIVE STUDY OF THEIR ISOLATION, EXPANSION AND CHARACTERISATION FROM THE BLOOD AND MATRIX OF THE UMBILICAL CORD
Journal Title: Stanley Medical Journal - Year 2014, Vol 1, Issue 1
Abstract
Mesenchymal stem cells (MSCs) are attractive tools for tissue engineering targeting clinical transplantation due to their regeneration potential and immuno-suppressive capacity. MSCs from bone marrow are widely used, but their harvest requires an invasive procedure. The umbilical cord which is discarded at birth, is a source for both cord blood as well as matrix (also known as Wharton’s jelly), which can be used to derive mesenchymal stem cells. We used both Umbilical cord blood (UCB) and Wharton’s Jelly (WJ ) to isolate MSCs by Density gradient separation and Explant culture method respectively. The same induction system (DMEM low glucose with 20% FBS) was used and the efficiency of isolation, proliferation and expansion were compared. The Wharton’s jelly derived MSC’s (WJMSCs) showed a relatively rapid proliferation and hence is a better source when compared to cord blood. The method of isolation of MSCs from Wharton’s jelly is non controversial, involves a painless procedure (compared to bone marrow aspiration, which is conventionally used) and relatively easier to process than cord blood. Hence Wharton’s jelly is a good source for MSCs with potential therapeutic applications in the future.
Authors and Affiliations
Rosy Vennila, Secunda R. , Rajasundari M. , Jeswanth S. , Surendran R.
Keywords
Related Articles
- EP ID EP217455
- DOI -
- Views 66
- Downloads 0
LOCAL SURGICAL RESECTION OF A CASE OF GASTRO INTESTINAL STROMAL TUMOR OF DUODENUM
Gastro intestinal stromal tumors of the duodenum are uncommon. We present the case of a 62-year-old woman with a gastrointestinal stromal tumor of the duodenum. Her complaint was melena. A local surgical resection of fir...
Burden of dengue fever and investigating challenges,Melur block,Tamilnadu,India
Introduction: Dengue has fast emerged to be an important public health problem for its ability to occur in large scale outbreaks .During the 1st week of October 2012, there were reports of rapid increase in number of fev...
A PROSPECTIVE STUDY ON ETIOLOGY AND MANAGEMENT OF OBSTRUCTIVE JAUNDICE DUE TO EXTRA HEPATIC BILIARY OBSTRUCTION
Jaundice or icterus a generic term used for yellowish discoloration of the skin, mucous membrane or sclera caused by a heterogeneous group of disorders. It is useful to divide the causes of obstructive jaundice into two...
HEMOPTYSIS -“EFFORT INDUCED THROMBOSIS”
A 30 year old male, photographer presented with the complaints of swelling and continuous throbbing pain in the right upper limb on the medial aspect for 2 weeks, cough with haemoptysis 3-4 episodes,each time 3-5 ml in t...
ADOLESCENT HEALTH AND HEALTHCARE DELIVERY IN INDIA : A REVIEW
Adolescence is a transition phase from childhood to adulthood characterised by rapid physical growth, social and psychological maturity and onset of reproduction cycle. Adolescents face widely pervasive health issues suc...