Human Mesenchymal Stem Cells Impregnated with Autologous Cartilage Paste Repair Fresh Focal Osteochondral Defects in Rabbits
Journal Title: International Journal of Medical Research Professionals - Year 2017, Vol 3, Issue 4
Abstract
Introduction: Several therapies have been attempted to treat focal osteochondral defects of the knee joint, but none of them resulted in full repair. We try in this study to determine the efficacy of human umbilical cord stem cell xenografts, on the healing of focal osteochondral cartilage defects, in rabbit's knee. Material and Methods: Focal defect was created in the medial femoral condyle of the right knee of all rabbit groups. In one group the defect was left untreated. Human umbilical cord mesenchmal stem cells (MSCs) were placed in the defect, in a second group. A third group received MSCs on a fibrin sealant scaffold. A fourth group received minced cartilage paste impregnated with MSCs. Healing was assessed clinically, radiologically and pathologically. Results: The animals were able to move normally after 8 weeks of surgery. Examination of the knee joints showed better healing of the defect in rabbits that received MSCs+ cartilage paste compared to other animal groups. Magnetic resonance imaging (MRI) showed persistent osteochondral defect in rabbits treated with MSCs, without and with fibrin sealant. Complete defect fill, intact cartilage surface, was observed in rabbits treated with MSCs impregnated with cartilage paste. Pathologic examination revealed that MSCs, alone, induced chondrocyte proliferation and stimulated cartilage repair. A better result was obtained with MSCs + fibrin sealant. The best repair was obtained when autologous cartilage paste was used. Conclusions: Repair of focal osteochondral defects in rabbit knees using human umbilical cord MSCs impregnated with autologous cartilage paste appears to be successful as proven clinically, radiologically, as well as pathologically.
Authors and Affiliations
Hazem Mahmoud Atta, Mohammad Mohammedaly Abbas, Mohammad Hussein Alkaf, Mamdouh M Kotb, Laila A Damiati, Gamal Karrouf, Soheir Mahfouz
Keywords
Related Articles
- EP ID EP546728
- DOI 10.21276/ijmrp.2017.3.4.021
- Views 118
- Downloads 0
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