IDENTIFICATION AND QUANTIFICATION OF OLEANOLIC ACID FROM CYCLEA PELTATA BY HIGH-PERFORMANCE THIN LAYER CHROMATOGRAPHY (HPTLC) METHOD
Journal Title: International Journal of Pharmaceutical Sciences and Research (IJPSR) - Year 2019, Vol 10, Issue 11
Abstract
To identify and quantify oleanolic acid from the roots of cyclea peltata by HPTLC method. Methods: The Plant cyclea peltata is a perennial herb from Menispermacae family. The powdered roots of plant cyclea peltata were extracted with methanol. Methanolic extract prepared separated with n-butanol, chloroform, and ethyl acetate as total saponin, total alkaloid and phenolic compound fractions respectively. The chemical characterization of oleanolic acid was done by High-Performance Thin Layer Chromatography (HPTLC) in a mixture of toluene: methanol (9:1) as a mobile phase which developed in anisaldehyde sulphuric acid reagent. Result: The extraction yield of total saponin fraction was 4.3% (w/w). The Rf value 0.31 found to same as the value of standard oleanolic acid. Linear regression analysis data for the calibration plots showed a good linear relationship between concentration (1-6 μg) R (r2) = 0.992408 concerning peak height and area. The limit of detection and limit of quantification of Oleanolic acid were detected to be 10.75 μg and 40.70 μg per spot. The amount of oleanolic acid in the methanol extract of roots was found to be 3.442 μg. Conclusion: Based on HPTLC result and its interpretation, it can be concluded that oleanolic acid present in roots of cyclea peltata which give identical, characteristic signals and absorbance similar to earlier reported reference standards.
Authors and Affiliations
H. D. Une et al.
Keywords
Related Articles
- EP ID EP665015
- DOI 10.13040/IJPSR.0975-8232.10(11).4899-06
- Views 88
- Downloads 0
EFFECT OF PHARMACIST DIRECTED COUNSELLING SERVICES ON KNOWLEDGE, ATTITUDE, AND PRACTICE (KAP) AND BLOOD PRESSURE CONTROL IN HYPERTENSIVE PATIENTS: A RANDOMIZED CONTROL TRIAL
Pharmacists play a vital role in providing counseling to the hypertensive patients to understand disease, risk factors, medications, and non-pharmacological measures required in controlling Blood Pressure (BP). This stud...
DISINFECTANTS, REGULATION AND REQUIREMENTS - GLOBAL OVERVIEW
Surface disinfectant is the multi-billion-dollar global industry with continuous growth opportunities linked to advancements in technology and innovations. However, innovations and new advancements do not always reach co...
EFFECT OF EDAPHIC FACTORS ON MAJOR SECONDARY METABOLITES OF TINOSPORA CORDIFOLIA AND NEEM GUDUCHI WITH RESPECT TO THEIR IMMUNOMODULATORY EFFECT
Tinospora cordifolia is a requisite medicinal plant, has been used for the treatment of various diseases and has been recommended for improving the immune system mentioned in Ayurveda and other systems of medicine as a m...
SELECTING PROTEIN STRUCTURE/S FOR DOCKING-BASED VIRTUAL SCREENING: A CASE STUDY ON TYPE II INHIBITORS OF VEGFR-2 KINASE
In this study, 36 crystal structures available with type I-V inhibitors of VEGFR-2 kinase in the RCSB PDB were classified into DFG-in/-out conformation using visual analysis and KLIFS database. The focus was on Type II i...
FORMULATION AND EVALUATION OF GASTRO RETENTIVE NOVEL FLOATING IN-SITU GELLING SYSTEM OF CURCUMIN
The objective of the present study was to formulate and evaluate a gastro-retentive stomach specific novel floating in-situ gelling system of curcumin for potentially treating gastric ulcer, associated with Helicobacter...