Identification of Cytochrmoe oxidase p450 in Heptocytes generated from in vitro differentiation of mouse mesenchymal stem cells
Journal Title: JOURNAL OF ADVANCES IN BIOLOGY - Year 2015, Vol 7, Issue 3
Abstract
This study was designed to identify the presence of cytochrome oxidase p450 (CYP3A4), in the hepatic cell lines which was generated from the in vitro differentiation of mouse bone marrow mesenchymal stem cells. Bone marrow meenchymal stem cells were first isolated by collecting both the femur and tibia of the mouse thigh bone, then exess tissue were removed and the cells were flushed from the bones and cultured under highly aseptic conditions. The isolated cells were characterized as a mesenchymal stem cells via using immunocytochemistry analysis. The characterized hepatocytes were subjected to a differentiation protocol in which the differentiation medium was contained a spesfic growth factor including (Hepatocytes growth factor (HGF), Fibroblast growth factor4 (FGF4), Oncostatin M and Dexamethasone, All theses growth factor was used for directing the BM-MSCs twards the hepatic line of cells. After three weeks of the differentiation protocol the diffrentation cells were examined for the presence of Cytochrome oxidase p450 (CYP3A4), which consider as one of the specific markers of hepatocytes. Identification eas done via two different methods first, via using the immunocytochemical analysis for the antihuman mouse CYP3A4 antibody and second by ELIZA test. The results showed a posotive reactivity of the differentiated cells towards the mouse antihuman CYP3A4 antibody as well as the eliza results showed a significant increase in the enzyme level of the cell lysate during the weeks of the differentiation experiment.
Authors and Affiliations
Zahraa K. Zedan, Shalal M. Hussain, Shahlaa M. Salih
Keywords
Related Articles
- EP ID EP654494
- DOI 10.24297/jab.v7i3.6551
- Views 193
- Downloads 0
Herbal Milliherb Development since 1999 - 2004-2014
The formula Milliherb consists of more than 25 herbs (similar effect) of approximately 70 % vegetable, fruit, spices and 30 % nontoxic Indonesian herbs. Milliherb Medicine that is efficacy similar or more than modern dru...
Antifungal Activities and Phytochemical Screening of Seeds, Leaves and Callus (hypocotyls and cotyledons ) Extracts of Jatropha curcas L.
The research was under taken to investigate the antifungal activity of seeds , leaves and callus extracts of Jatropha curcas L. , using methanol and petroleum ether solvents. Callus was induced from hypocotyls and cotyle...
ZJXG decoction promoted the fracture healing by reducing the decomposition of BMP-7, IGF-1, bFGF and TGFβ1 in fracture rats
The aim is to investigate the effect of Zhuang Jin Xu Gu (ZJXG) decoction on serum levels of bone morphogenetic protein-7 (BMP-7), insulin-like growth factor-1 (IGF-1), basic fibroblast growth factor (bFGF) and transform...
Population genetic structure of Epinephelus marginatus in the Central Mediterranean Sea (Gulf of Gabe¨s and the coast of Libya)
In the present study, mitochondrial DNA sequences from the cytochrome b (cytb) gene and seven nuclear microsatellites were examined to assess the genetic diversity of Epinephelus marginatus inhabiting Tunisian and Libyan...
HISTOLOGICAL AND STATISTICAL STUDIES ON THE OVARIAN CYCLE OF THE CARAMOTE PRAWN MELICERTUS KERATHURUS (FORSKAL, 1775) (CRUSTACEA DECAPODA)
The two ovaries of the caramote prawn Melicertus kerathurus lie above the hepatopancreas and below the pericardial sinus and the heart. Both ovaries were reduced in general size with white compact and sickle-shaped...