Identification of sequences that are differentially expressed in the Incompatible interaction Musa acuminata - Mycosphaerella fijiensis Morelet
Journal Title: Biotecnología Vegetal - Year 2008, Vol 8, Issue 1
Abstract
Black leaf streak disease caused by Mycosphaerella fijiensis Morelet is considered the most destructive and costly foliar disease of bananas and plantain. An important step for the elucidation of molecular mechanisms of the disease resistance is to know about genes involved in plant defense response to pathogens. Identification of differentially expressed sequences in resistant genotype ‘Calcutta 4’ (Musa acuminata, AA) at an early stage of infection with M. fijiensis (6 to 12 days post inoculation) from a suppressed subtractive library (SSH) was carried out. A number of 63 ESTs, which include 42 singletons and 21 contigs were obtained by assembling 97 sequences with CAP3 algorithm. Identification of sequences according to their homology with sequences stated at protein non-redundant database GenBank, allowed to gather them in six functional categories: protein destiny (1.6%), oxidative stress (4.8%), metabolism (6.3%), energy production (6.3%), unknown function (38.1%) and without homology (42.8%). Results obtained will contribute to a better understanding of pathosystem, which allow the design of new strategies related to genetic improvement of banana.
Authors and Affiliations
Milady Mendoza-Rodríguez , Aminael Sánchez-Rodríguez , Orelvis Portal , Mayra Acosta-Suárez , Berkis Roque , Monica Hofte , Elio Jiménez
Keywords
Related Articles
- EP ID EP683876
- DOI -
- Views 123
- Downloads 0
Effect of artificial inoculation of Mycosphaerella fijiensis on the induction of defence-related enzymes in two Musa genotypes
Black Sigatoka, caused by Mycosphaerella fijiensis Morelet (anamorph: Pseudocercospora fijiensis (Morelet) Deighton), is regarded as the most economically important foliar disease of bananas and plantains worldwide. Alth...
Field evaluation of sugarcane mutants of the variety ‘SP 70-1284’ obtained by in vitro mutagenesis
In the experimental area of the Campus Tabasco, km. 21, in the state of Tabasco, Mexico, seven mutants of sugarcane obtained from the application of Gamma radiation 60 Co were studied. The yield and its components and...
Translating theory into practice for conservation of germplasm of recalcitrant-seeded species
Recalcitrant seeds, which are metabolically-active and desiccation-sensitive, can be stored in the short-term at non-injurious temperatures under conditions precluding dehydration, but associated problems include fungal...
Plant regeneration through somatic embryogenesis in three ethiopian Coffea arabica Lin. hybrids
Breeders at Jimma Agricultural Research Center (JARC), Ethiopia, developed three arabica coffee hybrids: Aba Buna, Ghawe and MCH 2 that exhibited heterosis over their parents. However, their distribution to farmers had...
Mycobiota of donor plants and fungal contaminants from the in vitro establishment of bamboo species
The presence of fungal contaminants may limit the establishment of bamboos explants. Knowing the contaminant microorganisms present in donor plants allow to trace more appropriate control strategies. This work was aimed...