Immunohistochemical Approach to Diagnosis of Soft Tissue Tumors of Lower Extremities with its Possibilities and Limitations
Journal Title: International Journal of Medical Research Professionals - Year 2017, Vol 3, Issue 3
Abstract
Introduction: Soft tissue tumors have wide range of morphological differentiation. In many cases soft tissue tumors can be diagnosed by histopathological examination alone. In certain cases the confusing morphology, inspite of all efforts, leaves the pathologist uncertain about the exact nature of the neoplasm. For that reason we need the help of ancillary techniques like immunohistochemistry (IHC). Thus the purpose of our study was to evaluate usefulness of IHC in diagnosing soft tissue tumors with its limitations and we took only those cases which involved lower extremity as lower extremity is the commonest site of all soft tissue sarcomas of body. Method: Specific morphological patterns of soft tissue tumors were identified by hematoxylin and eosin (H&E) staining. IHC was done by different panels of antibodies according to the morphological patterns. Then comparison was made between H&E diagnosis and immunohistochemistry aided diagnostic method. Result: Out of 42 cases 28 cases were diagnosed by H&E and 14 cases were doubtful. IHC was helpful in diagnosing 38 cases with a z score 0f 2.69 and P value less than 0.01. Conclusion: The accurate diagnosis by immunohistochemistry depends on thorough clinical evaluation, precise histomorphological analysis followed by selection of proper antibody panel. There are few limitations of this ancillary technique which should not be over looked. A regular audit of whole of the procedures of IHC may help to identify the problem areas.
Authors and Affiliations
Barnali Maiti, Shubham Bhattacharya, Uma Banerjee, Subodh kumar Bhattacharya, Koyela Mondal
Keywords
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- EP ID EP548098
- DOI 10.21276/ijmrp.2017.3.3.050
- Views 77
- Downloads 0
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